DNA SEQUENCING BY SURFACE ENHANCED RAMAN DETECTION

通过表面增强拉曼检测进行 DNA 测序

• 批准号: 6182552
• 负责人: David Schultz
• 金额: $ 13.36万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6182552
• 关键词: DNA; Raman spectrometry; analytical method; biosensor device; electronic spectra; nanotechnology; nucleic acid quantitation /detection; nucleic acid sequence; nucleotides; technology /technique development; ultraviolet microscopy; ultraviolet spectrometry

DESCRIPTION (Adapted from the Investigator's Abstract): The overall objective is the development of a new innovative technology that will allow for the efficient sequencing of long stretches of unmodified DNA. Genomic sequence information will provide a concrete basis for understanding living organisms. Comparisons between the genomes of identical species will provide greater insight into the genetic basis of complex phenotypes, and the molecular pathways that lead to individual characteristics. In addition, the future availability of the complete human genome sequence from multiple individuals, will facilitate the development of a host of practical medical diagnostics and treatments, such as the identification of inherited genetic disorders, tests for cancer and related diseases, or genetic-based therapies. The resulting genomic sequence information will provide a crucial informational database that will improve the condition of human health, and catalyze both basic science research programs and functional studies at the genome level. The technology is based on the defection and recognition of the characteristic vibrational states (Raman spectrum) for each of the individual DNA bases, following prior separation by enzymatic digestion. The instrumentation and protocols under development will be capable of measuring the Raman spectrum for the spread-out series of individual isolated DNA bases, thereby identifying which of the DNA bases is present, as well as their correct sequential order.

描述（改编自研究者的摘要）：总体 目标是开发一种新的创新技术 将允许对长片段进行有效测序 未修饰的DNA。基因组序列信息将提供 理解生物体的具体基础。比较 相同物种的基因组之间的差异将提供更大的 深入了解复杂表型的遗传基础，以及 导致个体特征的分子途径。在 此外，完整人类基因组的未来可用性 来自多个个体的序列，将促进发展 一系列实用的医学诊断和治疗，例如 遗传性疾病的鉴定、癌症检测 和相关疾病，或基于基因的疗法。由此产生的 基因组序列信息将提供重要的信息 将改善人类健康状况的数据库，以及 促进基础科学研究计划和功能性科学研究计划 基因组水平的研究。该技术基于 特征振动状态的缺陷和识别 （拉曼光谱）对于每个单独的 DNA 碱基，如下 通过酶消化预先分离。仪器仪表和 正在开发的协议将能够测量拉曼 单个分离 DNA 的展开系列光谱 碱基，从而识别存在哪些 DNA 碱基，如 以及它们的正确顺序。