DNA SEQUENCING BY SURFACE ENHANCED RAMAN DETECTION

通过表面增强拉曼检测进行 DNA 测序

• 批准号: 2822798
• 负责人: David Schultz
• 金额: $ 13.33万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2822798
• 关键词: DNA; Raman spectrometry; analytical method; biosensor device; electronic spectra; nanotechnology; nucleic acid quantitation /detection; nucleic acid sequence; nucleotides; technology /technique development; ultraviolet microscopy; ultraviolet spectrometry

DESCRIPTION (Adapted from the Investigator's Abstract): The overall objective is the development of a new innovative technology that will allow for the efficient sequencing of long stretches of unmodified DNA. Genomic sequence information will provide a concrete basis for understanding living organisms. Comparisons between the genomes of identical species will provide greater insight into the genetic basis of complex phenotypes, and the molecular pathways that lead to individual characteristics. In addition, the future availability of the complete human genome sequence from multiple individuals, will facilitate the development of a host of practical medical diagnostics and treatments, such as the identification of inherited genetic disorders, tests for cancer and related diseases, or genetic-based therapies. The resulting genomic sequence information will provide a crucial informational database that will improve the condition of human health, and catalyze both basic science research programs and functional studies at the genome level. The technology is based on the defection and recognition of the characteristic vibrational states (Raman spectrum) for each of the individual DNA bases, following prior separation by enzymatic digestion. The instrumentation and protocols under development will be capable of measuring the Raman spectrum for the spread-out series of individual isolated DNA bases, thereby identifying which of the DNA bases is present, as well as their correct sequential order.

描述（改编自研究者摘要）：总体 目标是开发一种新的创新技术， 将允许对长时间的 未经修饰的DNA基因组序列信息将提供 理解生物体的具体基础。比较 相同物种的基因组之间的差异， 深入了解复杂表型的遗传基础， 导致个体特征的分子途径。在 此外，未来完整的人类基因组的可用性 从多个人的序列，将促进发展 一系列实用的医疗诊断和治疗方法，如 遗传性疾病的鉴定，癌症的检测 和相关疾病，或基于基因的疗法。所得 基因组序列信息将提供重要的信息 改善人类健康状况的数据库，以及 促进基础科学研究计划和功能 基因组水平的研究。该技术基于 特征振动态的检测与识别 （拉曼光谱）， 然后通过酶消化进行分离。仪表和 正在开发的协议将能够测量的拉曼 单个分离DNA的展开系列的光谱 碱基，从而鉴定存在哪些DNA碱基，如 以及它们的正确顺序。