CORE--BIOCHEMISTRY AND MOLECULAR BIOLOGY

生物化学和分子生物学(BMB)核心将提供技术 和人员支持，以促进、规范和实施 分析组织样本，并将在#年为SCOR的调查人员提供支持 使用新的生化分析、免疫化学和分子生物学 技巧。这一核心设施将通过其所有单位为 需要协助(1)进行抗体工作的调查人员包括 所有类型的免疫化学方法，并生产新的单抗 或多克隆抗体，(2)在定位不同的基因(MRNAs)中 组织切片或细胞培养，以及(3)所有类型的分子生物学 方法：研究方法。BMB核心计划通过以下方式帮助每个SCOR项目 技术、仪器和人员支持(一)常用的 由几个项目或(Ii)调查人员偶尔使用，但需要 特殊技术或安全法规或(三)需要特殊设备 或技术专长。此外，这个核心将引入、建立 并为调查人员提供新的方法，如果个别SCOR项目 没有所需的技术或人员支持或特殊专业知识 建立一种新的但耗时的方法。BMB核心设施，总部位于 浅谈当前SCOR拨款的经验和预期需求 本申请由三个主要单元组成。他们 杂交瘤/免疫化学核心单位将容纳所有 与特定的单克隆体或多克隆体的发育有关 抗体和/或是识别、检测或 使用抗体对蛋白质进行量化。因此，这个核心将服务于所有 希望检测或量化软骨基质分子或 它们在血清、尿液、组织培养液或组织中的“代谢物” 萃取物。原位杂交核心单位将支持所有研究 需要在组织切片或组织切片中定位各种mRNA 细胞培养。因此，这一核心设施将帮助 对正常的细胞或组织进行专门的基因分析处理 或者病理样本。分子生物学核心单位将提供 在整个分子生物学曲目范围内的专业知识。这个核心 该股将在一个更专门的领域提供帮助和专业知识 分子医学和遗传学。因为这些技术中有许多需要 不同方法和协议的频繁组合，我们打算 将这些核心单位集中到一个核心设施中 由一个人协调，确保最有效和最高效的 研究方法与质量控制评估相结合。

The Biochemistry and Molecular Biology (BMB) Core will provide technical and personnel support to facilitate, standardize and implement the analysis of tissue samples and will support investigators of the SCOR in using new biochemical assays, immunochemical and molecular biology techniques. This Core facility will serve through its units all investigators who will require assistance (1) with antibody work including all types of immunochemical methods, and the production of new monoclonal or polyclonal antibodies, (2) in localizing various genes (mRNAs) in tissue sections or cell cultures, and (3) all types of molecular biology methods. The BMB Core intends to aid each SCOR project by providing technical, instrumental and personnel support (i) which are commonly used by several projects or (ii) occasionally used by investigators but require special technical or safety regulations or (iii) require special equipment or technical expertise. Furthermore, this core will introduce, establish and provide new methods to investigators, if the individual SCOR projects do not have technical or personnel support or special expertise needed to establish a new, but time-consuming, method. The BMB Core facility, based on the experience of the current SCOR grant and the expected demands of this application, consists of three major units. They Hybridoma/Immunochemistry Core Unit will accommodate all investigators who are concerned with the development of specific monoclonal or polyclonal antibodies and/or are facilitating methods to identify, detect, or quantify proteins using antibodies. Thus, this core will serve all investigators who want to detect or quantify cartilage matrix molecules or their "metabolites" in sera, urine, tissue culture media or tissue extracts. The in situ Hybridization Core Unit will support all studies requiring the localization of various mRNAs either in tissue sections or cell cultures. Thus, this core facility will help investigators in the processing of cells or tissues for specialized analysis of genes in normal or pathological samples. The Molecular Biology Core Unit will provide expertise in the entire scale of molecular biology repertoire. This core unit will help and provide expertise in a more specialized area of molecular medicine and genetics. Since many of these techniques require frequent combination of different methods and protocols, we intend to centralize these core units into one Core facility directed and coordinated by one person, to guarantee the most effective and efficient integration of research approaches and assessment of quality control.