RENAL TRANPORT OF NA+, K+, CL- AND H+

离子跨肾小管转运的机制与调控 我们的研究项目旨在澄清尚未解决的 跨顶侧和基底侧的离子运输过程的问题 哺乳动物肾单位的近端和远端肾单位节段的膜。 我们将应用小管灌注方法，探索离子电导 和天然离子通道的电生理技术， 用荧光计和电子探针测量细胞离子浓度 接近。我们还将合作研究克隆CI- 草酸盐和Cl-甲酸盐交换剂。我们建议利用几个 遗传性小鼠动物模型，其中离子载体是 过度表达或缺失。我们的重点将是钠，钾，氯和氢的运输 机制及其生理调节。我们还将调查 主要的小管细胞信号通路，协调顶端和 基底外侧转运过程。

MECHANISM AND REGULATION OF ION TRANSPORT ACROSS THE RENAL TUBULE Our research project is directed toward clarification of unresolved problems of ion transport processes across the apical and basolateral membranes of proximal and distal nephron segments of mammalian nephrons. We shall apply tubule perfusion methods, exploration of ion conductances and of native ion channels by electrophysiological techniques, and measurements of cell ion concentrations by fluorometric and electron probe approaches. We will also collaborative in studies of cloning the CI- oxalate and CI-formate exchanger. We propose to take advantage of several hereditary mouse animal models in which ion carriers are either overexpressed or absent. Our focus will be on Na, K, CI and H transport mechanisms and their physiological regulation. We shall also investigate principle tubule cell signaling pathways that coordinate apical and basolateral transport processes.