RENAL TRANPORT OF NA+, K+, CL- AND H+

NA、K、CL- 和 H 的肾转运

• 批准号: 6412907
• 负责人: GERHARD H GIEBISCH
• 金额: $ 14.86万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-12-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6412907
• 关键词: Anura; acid base balance; apical membrane; basolateral membrane; bicarbonates; chloride ion; electron probe spectrometry; formates; gene targeting; genetically modified animals; hormone regulation /control mechanism; hydrogen ions; ion transport; laboratory mouse; laboratory rabbit; laboratory rat; membrane transport proteins; molecular cloning; oxalates; perfusion; potassium ion; renal tubular transport; sodium ion; sodium potassium exchanging ATPase; voltage /patch clamp

MECHANISM AND REGULATION OF ION TRANSPORT ACROSS THE RENAL TUBULE Our research project is directed toward clarification of unresolved problems of ion transport processes across the apical and basolateral membranes of proximal and distal nephron segments of mammalian nephrons. We shall apply tubule perfusion methods, exploration of ion conductances and of native ion channels by electrophysiological techniques, and measurements of cell ion concentrations by fluorometric and electron probe approaches. We will also collaborative in studies of cloning the CI- oxalate and CI-formate exchanger. We propose to take advantage of several hereditary mouse animal models in which ion carriers are either overexpressed or absent. Our focus will be on Na, K, CI and H transport mechanisms and their physiological regulation. We shall also investigate principle tubule cell signaling pathways that coordinate apical and basolateral transport processes.

肾小管离子转运的机制和调控