RENAL TRANPORT OF NA+, K+, CL- AND H+

NA、K、CL- 和 H 的肾转运

• 批准号: 6201822
• 负责人: GERHARD H GIEBISCH
• 金额: $ 14.86万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6201822
• 关键词: Anura; acid base balance; apical membrane; basolateral membrane; bicarbonates; chloride ion; electron probe spectrometry; formates; gene targeting; genetically modified animals; hormone regulation /control mechanism; hydrogen ions; ion transport; laboratory mouse; laboratory rabbit; laboratory rat; membrane transport proteins; molecular cloning; oxalates; perfusion; potassium ion; renal tubular transport; sodium ion; sodium potassium exchanging ATPase; voltage /patch clamp

MECHANISM AND REGULATION OF ION TRANSPORT ACROSS THE RENAL TUBULE Our research project is directed toward clarification of unresolved problems of ion transport processes across the apical and basolateral membranes of proximal and distal nephron segments of mammalian nephrons. We shall apply tubule perfusion methods, exploration of ion conductances and of native ion channels by electrophysiological techniques, and measurements of cell ion concentrations by fluorometric and electron probe approaches. We will also collaborative in studies of cloning the CI- oxalate and CI-formate exchanger. We propose to take advantage of several hereditary mouse animal models in which ion carriers are either overexpressed or absent. Our focus will be on Na, K, CI and H transport mechanisms and their physiological regulation. We shall also investigate principle tubule cell signaling pathways that coordinate apical and basolateral transport processes.

离子跨肾小管转运的机制及调控 我们的研究项目旨在澄清尚未解决的问题 跨越根尖和基底外侧的离子传输过程问题 哺乳动物肾单位近端和远端肾单位的膜。 我们将应用小管灌注法，离子电导的探测 以及通过电生理技术对天然离子通道进行研究，以及 用荧光法和电子探针法测量细胞离子浓度 接近了。我们还将合作研究克隆CI- 草酸盐和CI-甲酸盐交换剂。我们建议利用几个 遗传性小鼠动物模型，其中离子载体 过度表达或缺席。我们的重点将放在Na、K、CI和H的运输上 其作用机制及其生理调控。我们还将调查 主要的小管细胞信号通路协调心尖和 基底侧向运移过程。