ACQUISITION OF BRUKER DALTONICS REFLEX III MALDI TOF

收购 BRUKER DALTONICS REFLEX III MALDI TOF

• 批准号: 2791793
• 负责人: JOHN D LAMBRIS
• 金额: $ 25万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2791793
• 关键词: biomedical equipment; biomedical equipment purchase; matrix assisted laser desorption ionization

The specific aim of this application is to acquire a state-of-the-art high performance MALDI-TOF mass spectrometer at the University of Pennsylvania to meet the needs of researchers concerned with studies of proteins, peptides, and other biomolecules. Specifically, the proposal intent is to expand the capabilities of the existing Protein Chemistry Laboratory (PCL) in the School of Medicine. The core facility is a state of art microchemical core facility offspring, to the entire University, the following services: 1) peptide synthesis, purification, and immunogenic coupling 2) peptide modifications 3) protein purification 4) tryptic peptide preparations 5) protein microsequencing 6.) mass spectroscopy and 7) training in protein purification and analysis. This instrument will enable us to a) obtain highly accurate molecular weight data on low pmole to femtomole amounts of peptides and proteins and permit rapid mass mapping identification of proteins whose cDNA-derived sequence information exists in the ever expanding databases. This approach is becoming essential to the management of protein sequencing resources available in an all-encompassing protein core laboratory like the PCL. Post source decay (PSD) and Collisionally induced dissociation (CID) features coupled with accurate mass data will provide partial sequence information on peptides even if present in a mixtures of N- terminally blocked. Structural problems on proteins and other biomolecules difficult or not approachable with conventional techniques will be addressable. Significantly the instrument will be integrated into all aspects of protein chemistries performed at the PCL such as Edman sequencing and peptide synthesis projects. In summary the new instrumentation will provide high-level-of-confidence answers for problems involving, natural product and expression product analysis, protein design, peptide synthesis, amino acid sequencing, ligand identification, post-translational modification identification., and protein identification through accurate mass mapping, characterization of non-protein biomolecules. We have identified a core of 10 major user research projects, from departments spanning four Penn schools (Art & Sciences, Dental Medicine, Veterinary Medicine and Medicine). The areas of interest include, protein-protein interactions, phylogeny of immune system, gene expression and protein design, anesthesia-related chemical modification of proteins, disulfide linkage determination, and biology of cell-growth and differentiation. Several people have already benefitted from interaction with our core and the new instrumentation will enhance the quality and quantify of results connected with future investigations of the various participating laboratories.

这项应用的具体目标是在宾夕法尼亚大学获得最先进的高性能MALDI-TOF质谱仪，以满足关注蛋白质、多肽和其他生物分子研究的研究人员的需求。具体地说，该提案的目的是扩大医学院现有蛋白质化学实验室(PCL)的能力。核心设施是一个最先进的微化学核心设施的后代，为整个大学提供以下服务：1)肽合成、纯化和免疫原性偶联2)肽修饰3)蛋白质纯化4)胰蛋白酶多肽制剂5)蛋白质微测序6)质谱学以及蛋白质纯化和分析方面的培训。该仪器将使我们能够a)获得从低分子到毫微摩尔量的高精度的多肽和蛋白质的分子量数据，并允许快速的质谱图识别其cDNA衍生序列信息存在于不断扩大的数据库中的蛋白质。这种方法对于管理像PCL这样包罗万象的蛋白质核心实验室中可用的蛋白质测序资源正变得至关重要。源后衰减(PSD)和碰撞诱导解离(CID)特征与准确的质量数据相结合，即使存在于N-末端被阻断的混合物中，也可以提供关于肽的部分序列信息。蛋白质和其他生物分子上的结构问题将是可以解决的，用传统技术很难或无法接近这些问题。值得注意的是，该仪器将集成到PCL进行的蛋白质化学的所有方面，如Edman测序和多肽合成项目。总之，新的仪器将为涉及天然产物和表达产物分析、蛋白质设计、肽合成、氨基酸测序、配体鉴定、翻译后修饰鉴定以及通过准确的质量映射、非蛋白质生物分子表征的蛋白质鉴定等问题提供高水平的置信度答案。我们已经确定了10个主要用户研究项目的核心，这些项目来自宾夕法尼亚大学四所学校(艺术与科学、牙科医学、兽医和医学)的部门。感兴趣的领域包括蛋白质-蛋白质相互作用，免疫系统的系统发育，基因表达和蛋白质设计，麻醉相关的蛋白质化学修饰，二硫键连接测定，以及细胞生长和分化的生物学。一些人已经从与我们的核心的互动中受益，新的仪器将提高与各参与实验室未来调查有关的结果的质量和数量。