PURCHASE OF AN LC/MS/MS INSTRUMENT

这项申请申请资金，以允许购买LC-MS-MS仪器，这将为爱荷华大学的研究人员提供共享质谱学能力的重大升级。拟议中的仪器将设在爱荷华大学高分辨率质谱学设施(UIHRMSF)，并将由一名博士级别的质谱学专家操作和维护。所要求的系统包括一台能够进行串联质谱仪(MS-MS)实验的四极离子陷阱质谱仪，并将与一台液体色谱仪(LC)对接。电喷雾电离(ESI)和常压化学电离(APCI)将作为电离方法。样品可以通过LC或使用双注射器泵直接输液来引入。目前，校园内有44个研究小组使用UIHRMSF，其中近四分之三参与了生物医学研究。MS-MS功能在园区中不可用，并且此应用程序中列出的主要用户组都无法访问LC-MS服务。拟议的仪器将配置为主要用户开放使用的系统。这份提案描述了NIH支持的七个主要用户群体的项目，尽管校园里的其他生物医学研究人员也将可以使用该仪器。这些项目包括：真菌天然产物的结构测定，新型核苷膦的表征，酶修饰的分析，中性和硫化低聚糖的序列分析，修饰的血红素的结构测定，泛素中18O-掺入的分析，氧化脂质代谢物的鉴定，蛋白质类抗肿瘤药物的结构研究和蛋白质中2H/1H交换位点的鉴定。获得拟议仪器提供的最先进的质谱学能力将有利于所有这些研究计划。

This application requests funding to permit the purchase of an LC-MS-MS instrument that would provide a significant upgrade of the shared mass spectrometry capabilities available to researchers at the University of Iowa. The proposed instrument will be located in the University of Iowa High Resolution Mass Spectrometry Facility (UIHRMSF), and will be operated and maintained by a Ph.D.-level mass spectrometry specialist. The requested system consists of a quadrupole ion trap mass spectrometer capable of performing tandem mass spectrometry (MS-MS) experiments, and will be interfaced with a liquid chromatograph (LC). Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) will be available as ionization methods. Samples can be introduced either by LC or by direct infusion using a dual syringe pump. Currently, there are 44 research groups on campus that utilize the UIHRMSF, and nearly three-quarters of these are involved in biomedical research. MS-MS capability is not available on campus, and none of the major user groups listed in this application have access to LC-MS service. The proposed instrument will be configured for use as an open access system for the major users. Projects from seven NIH-supported major users groups are described in this proposal, although other biomedical researchers on campus will also have access to the instrument. These projects include: structure determination of natural products from fungi, characterization of novel nucleoside phosphonates, analysis of enzyme modifications, sequence analysis of neutral and sulfated oligosaccharides, structure determination of modified hemes, analysis of 18O-incorporation in ubiquitin, identification of oxygenated lipid metabolites, investigation of the structure of a proteinaceous anti-tumor agent and identification of 2H/1H exchange sites in proteins. Access to the state-of-the-art mass spectrometry capabilities provided by the proposed instrument would be beneficial to all of these research programs.