Acquisition of a Q-TOF Mass Spectrometer

购买 Q-TOF 质谱仪

• 批准号: 7211166
• 负责人: JAMES B GLOER
• 金额: $ 48.74万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-15 至 2008-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7211166
• 关键词: Acceleration; Antimalarials; Antineoplastic Agents; Arts; Atmospheric Pressure; Biological Factors; Biomedical Research; Chemicals; Communicable Diseases; Development; Dopamine; Drug Addiction; Equipment; Funding; Future; Hybrids; Investigation; Iowa; Ligands; Liquid Chromatography; Mass Spectrum Analysis; Microfilaments; Modification; Molecular Conformation; Myopathy; Opioid; Parkinson Disease; Peptides; Periodontal Diseases; Protein Analysis; Proteins; Request for Applications; Research; Research Personnel; Resolution; Site; Support of Research; Terpenes; Time; Universities; antimicrobial peptide; experience; fungus; improved; instrument; instrumentation; ionization technique; mass spectrometer; member; nano-electrospray; novel; phosphonate; programs; research study; synthetic protein; tandem mass spectrometry

DESCRIPTION (provided by applicant): This application requests funding to permit the purchase of a new mass spectrometer that would provide a significant upgrade of the shared instrumentation capabilities available to researchers at the University of Iowa. The proposed instrument will be located in the University of Iowa High Resolution Mass Spectrometry Facility (UIHRMSF), and will be operated and maintained by experienced staff members. The requested instrument is a hybrid quadrupole/orthogonal acceleration time-of-flight (oa- TOF) mass spectrometer capable of performing high-resolution mass spectrometry (HRMS) and HR tandem mass spectrometry (MS/MS) experiments, and will have a liquid chromatography interface. The instrument will be capable of analysis using electrospray, nano-electrospray, and atmospheric pressure chemical ionization methods. Over forty research groups on campus utilize the UIHRMSF, and over thirty of these are involved in biomedical research. The proposed instrument was selected to fulfill current and future needs of the major NIH-sponsored users, as well as other biomedical researchers on campus. Ten NIH-supported projects from eight major users groups described in this proposal provide justification for the acquisition of the proposed mass spectrometer. These projects include structural studies of bioactive natural products from fungi; characterization of novel opioid ligands and their target proteins; analysis of proteins modified by a dopamine metabolite (DOPAL); characterization of novel terpenoid phosphonates and their interactions with RAS proteins; investigation of actin filament conformation using H/D exchange studies; characterization of PEG-peptide conjugates and identification of modification sites in synthetic protein conjugates; synthesis and characterization of natural products and related compounds as potential anticancer and antimalarial agents; and identification of naturally occurring antimicrobial peptides. Access to the state-of-the-art mass spectrometry capabilities of the proposed instrument would be beneficial to all of these research programs. Relevance. The equipment requested in this proposal will be used to support research directed toward the discovery and development of new treatments for cancer, drug dependency, and infectious diseases. Projects dedicated to improving our understanding of periodontal disease, Parkinson's Disease, and various muscular disorders will also benefit from the capabilities of this instrument.

描述（由申请人提供）：本申请申请资金用于购买一台新的质谱仪，该质谱仪将为爱荷华大学的研究人员提供共享仪器功能的重大升级。拟议的仪器将位于爱荷华大学高分辨率质谱设施（uihrrmsf），并将由经验丰富的工作人员操作和维护。所要求的仪器是一台混合四极杆/正交加速飞行时间（oa- TOF）质谱仪，能够进行高分辨率质谱（HRMS）和高分辨率串联质谱（MS/MS）实验，并将具有液相色谱接口。该仪器将能够使用电喷雾、纳米电喷雾和大气压化学电离方法进行分析。校园内有40多个研究小组利用该基金，其中30多个涉及生物医学研究。所建议的仪器被选择来满足当前和未来的需要，主要的nih赞助用户，以及其他生物医学研究人员在校园。本提案中描述的来自8个主要用户组的10个nih支持的项目为购买提议的质谱仪提供了理由。这些项目包括真菌生物活性天然产物的结构研究；新型阿片配体及其靶蛋白的表征多巴胺代谢物（DOPAL）修饰蛋白的分析；新型萜类膦酸盐的表征及其与RAS蛋白的相互作用；H/D交换研究肌动蛋白丝构象；聚乙二醇肽偶联物的鉴定和合成蛋白偶联物修饰位点的鉴定；作为潜在抗癌和抗疟药物的天然产物及相关化合物的合成和表征以及天然抗菌肽的鉴定。获得所提议的仪器的最先进的质谱分析能力将有利于所有这些研究项目。的相关性。本提案所要求的设备将用于支持针对癌症、药物依赖和传染病的新疗法的发现和开发的研究。致力于提高我们对牙周病、帕金森病和各种肌肉疾病的了解的项目也将受益于该仪器的功能。

项目成果

Bioactive natural products from fungicolous Hawaiian isolates: secondary metabolites from a Phialemoniopsis sp.

来自夏威夷真菌分离物的生物活性天然产物：来自Phalemoniopsis sp.的次生代谢物。

• DOI: 10.1080/21501203.2014.931309
• 发表时间: 2014
• 期刊: Mycology
• 作者: Kaur,Amninder;Rogers,KristinaD;Swenson,DaleE;Dowd,PatrickF;Wicklow,DonaldT;Gloer,JamesB
• 通讯作者: Gloer,JamesB

Broomeanamides: Cyclic Octapeptides from an Isolate of the Fungicolous Ascomycete Sphaerostilbella broomeana from India.

• DOI: 10.1021/acs.jnatprod.1c00414
• 发表时间: 2021-07-23
• 期刊: Journal of natural products
• 影响因子: 5.1
• 作者: Ekanayake DI;Perlatti B;Swenson DC;Põldmaa K;Bills GF;Gloer JB
• 通讯作者: Gloer JB

Disseminins and Spiciferone Analogues: Polyketide-Derived Metabolites from a Fungicolous Isolate of Pestalotiopsis disseminata.

• DOI: 10.1021/acs.jnatprod.5b00907
• 发表时间: 2016-03
• 期刊: Journal of natural products
• 影响因子: 5.1
• 作者: In Hyun Hwang;D. Swenson;J. Gloer;D. Wicklow

Aflaquinolones A-G: secondary metabolites from marine and fungicolous isolates of Aspergillus spp.

• DOI: 10.1021/np200958r
• 发表时间: 2012-03-23
• 期刊: Journal of natural products
• 影响因子: 5.1
• 作者: Neff SA;Lee SU;Asami Y;Ahn JS;Oh H;Baltrusaitis J;Gloer JB;Wicklow DT
• 通讯作者: Wicklow DT