Acquisition of a Q-TOF Mass Spectrometer

描述(由申请者提供)：本申请申请资金，以允许购买新的质谱仪，这将为爱荷华大学的研究人员提供共享仪器能力的重大升级。拟议的仪器将设在爱荷华大学高分辨率质谱学设施(UIHRMSF)，并将由经验丰富的工作人员操作和维护。所要求的仪器是能够进行高分辨率质谱学(HRMS)和高分辨率串联质谱学(MS/MS)实验的混合四极杆/正交加速飞行时间质谱仪，并将具有液相色谱接口。该仪器将能够使用电喷雾、纳米电喷雾和大气压化学电离方法进行分析。校园内有40多个研究小组使用UIHRMSF，其中30多个小组参与了生物医学研究。建议的仪器是为了满足NIH赞助的主要用户以及校园内其他生物医学研究人员的当前和未来需求而选择的。本提案中描述的来自8个主要用户组的10个NIH支持的项目为采购拟议的质谱仪提供了理由。这些项目包括：真菌生物活性天然产物的结构研究；新型阿片配体及其靶蛋白的表征；多巴胺代谢物修饰的蛋白质的分析；新型萜类磷酸盐的表征及其与RAS蛋白的相互作用；利用H/D交换研究肌动蛋白细丝构象；聚乙二醇肽结合物的表征以及合成蛋白质结合物中修饰位点的确定；作为潜在抗癌和抗疟疾药物的天然产品和相关化合物的合成和鉴定；以及天然抗菌肽的鉴定。获得拟议仪器的最先进的质谱学能力将有利于所有这些研究计划。关联性。该提案中要求的设备将用于支持旨在发现和开发治疗癌症、药物依赖和传染病的新疗法的研究。致力于提高我们对牙周病、帕金森氏病和各种肌肉疾病的了解的项目也将受益于该仪器的能力。

DESCRIPTION (provided by applicant): This application requests funding to permit the purchase of a new mass spectrometer that would provide a significant upgrade of the shared instrumentation capabilities available to researchers at the University of Iowa. The proposed instrument will be located in the University of Iowa High Resolution Mass Spectrometry Facility (UIHRMSF), and will be operated and maintained by experienced staff members. The requested instrument is a hybrid quadrupole/orthogonal acceleration time-of-flight (oa- TOF) mass spectrometer capable of performing high-resolution mass spectrometry (HRMS) and HR tandem mass spectrometry (MS/MS) experiments, and will have a liquid chromatography interface. The instrument will be capable of analysis using electrospray, nano-electrospray, and atmospheric pressure chemical ionization methods. Over forty research groups on campus utilize the UIHRMSF, and over thirty of these are involved in biomedical research. The proposed instrument was selected to fulfill current and future needs of the major NIH-sponsored users, as well as other biomedical researchers on campus. Ten NIH-supported projects from eight major users groups described in this proposal provide justification for the acquisition of the proposed mass spectrometer. These projects include structural studies of bioactive natural products from fungi; characterization of novel opioid ligands and their target proteins; analysis of proteins modified by a dopamine metabolite (DOPAL); characterization of novel terpenoid phosphonates and their interactions with RAS proteins; investigation of actin filament conformation using H/D exchange studies; characterization of PEG-peptide conjugates and identification of modification sites in synthetic protein conjugates; synthesis and characterization of natural products and related compounds as potential anticancer and antimalarial agents; and identification of naturally occurring antimicrobial peptides. Access to the state-of-the-art mass spectrometry capabilities of the proposed instrument would be beneficial to all of these research programs. Relevance. The equipment requested in this proposal will be used to support research directed toward the discovery and development of new treatments for cancer, drug dependency, and infectious diseases. Projects dedicated to improving our understanding of periodontal disease, Parkinson's Disease, and various muscular disorders will also benefit from the capabilities of this instrument.