600MHZ WIDE BORE NMR SPECTROMETER

该赠款提案要求为600 MHz宽口径NMR光谱仪提供部分资金支持。该仪器将位于NMR研究中心，作为整合生物医学专用NMR仪器和促进合作研究的整体战略的一部分。所要求的光谱仪将满足一个主要用户群体对体内显微成像和光谱学日益增长的需求，而现有仪器无法满足这些需求。一个主要的用户组，包括八个独立的研究项目被确定，和组的成员，或期望在授予时，同行收到NIH的支持。在提案研究计划的导言部分以及个别研究项目说明中讨论了拟议光谱仪的理由和利用。该提案的主要优势来自各个研究项目本身，所有这些项目都具有临床和生物医学相关性。阿尔茨海默氏病将使用互补的转基因小鼠模型（Caserta）和结构肽分析（Krafft）进行机制研究。在两个项目（Groothuis和Wyrwicz）中讨论了中枢神经系统内的药物递送和药代动力学。Lurie综合癌症中心的合作者有研究乳腺癌（Thor）致癌的项目，结肠癌（Knop）治疗失败的原因以及肿瘤血管生成领域的主要贡献者（Bouck和Soff）的两个项目。这种广泛的代表性在西北大学家庭集中在神经科学和癌症领域是无与伦比的任何其他科学财团在芝加哥大都市区。该提案的另一个优势是核磁共振研究中心提供的多个专业的专业知识，并获得成功实施实验策略所需的资源支持。

This grant proposal requests partial funding support for a 600 MHz wide bore NMR spectrometer. The instrument will be located at the Center for NMR Research as part of an overall strategy to consolidate biomedically dedicated NMR instruments and promote collaborative research. The requested spectrometer will accommodate the growing needs of a major user group for in vivo microimaging and spectroscopy that cannot be met with the existing instrumentation. A major user group comprising eight separate research projects is identified, and members of the group have, or expect to have at the time of the grant award, peer-received NIH support. Justification and utilization of the proposed spectrometer are discussed in the introductory section of the research plan of the proposal, as well as within the individual research project description. The major strength of the proposal comes from the individual research projects themselves, all with clinical and biomedical relevance. Alzheimer's disease will be investigate mechanistically using both transgenic mouse models (Caserta) and structural peptide analysis (Krafft) which are complementary. Drug delivery and pharmacokinetics within the central nervous system are addressed in two projects (Groothuis and Wyrwicz). The Lurie Comprehensive Cancer Center collaborators have projects which investigate the carcinogenesis of breast cancer (Thor), causes of treatment failures in colon cancer (Knop) and two projects from major contributors to the field of tumor angiogenesis (Bouck and Soff). This broad representation within the Northwestern University Family focused on the areas of neuroscience and cancer is unmatched by any other scientific consortium in the Chicago metropolitan area. An additional strength of the proposal is the expertise of the multiple specialties available through the Center for NMR Research, and supported with resources necessary for successful implementation of experimental strategies.