TRANSMISSION ELECTRON MICROSCOPE--PHILIPS CM120

一组14名调查人员要求资金购买一个高 分辨率，计算机控制的菲利普斯CM 120透射电子 显微镜（TEM）。13名用户目前拥有NIH的支持， 有NSF/海军的支持。该组织建议取代目前唯一的 细胞生物学与解剖学系的TEM。的 15岁的JEOL 200 CX已经有了越来越多的停机时间， 近五年来，缺少许多关键配件，并有一些标准 不太适合研究生物标本的特征。 例如，200 CX设计用于高对比度下的低对比度操作， 加速电压高达200 kV，这为以下方面提供了一些优势： 研究厚标本例如，200 CX专为低 在高达200 kV的高加速电压下的对比度操作， 为研究厚标本提供了一些优势。比如说 200 CX是为高加速下的低对比度操作而设计的 电压高达200千伏，这为研究厚 标本年，对该功能的需求大幅下降。 近年来，重要的是，设计已经损害了操作， 低倍率和标准电压。此外，TEM的需要 在过去五年中， 200 CX的调度和性能。所要求的文书将 克服了现有TEM的许多当前问题， 提高效率和图像质量的关键功能 制作。此外，所要求的配件将使该集团 进行新型实验的能力目前还不可能。 许多学生要求考试的标本相对较低 固有的对比度，例如带有金抗体复合物的标本。的 菲利普斯CM 120的专利双物镜透镜尤其 设计用于在低放大倍数下检查生物样本， 高对比度和宽视野。高分辨率底部安装 CCD数码相机附件将允许收集数字化图像 用于在线傅立叶分析、计算机断层扫描、自动 剪辑、低/最小剂量成像和出版物制作 高质量的印刷品，从而在许多应用中取代传统的胶片。 这些和其他功能非常适合多学科 需要TEM的项目。该工具将大大提高 生产力的一大群经验丰富的科学家，九个从 细胞生物学与解剖学系和其他五个部门在 大学，并显着改善微观设施， 社会各界

A group of fourteen investigators requests funds to purchase a high resolution, computer controlled Phillips CM120 transmission electron microscope (TEM). Thirteen of the users have current NIH support and one has NSF/Navy support. The group proposes to replace the only currently available TEM within the Department of Cell Biology & Anatomy. The fifteen-year-old JEOL 200CX has had progressively more downtime in the last five years, lack many key accessories, and has some standard features that are not well-suited for studying biological specimens. For example, the 200 CX was designed for low contrast operation at high accelerating voltages up to 200 kV, which offers some advantages for studying thick specimens. For example, the 200 CX was designed for low contrast operation at high accelerating voltage up to 200 kV, which offers some advantages for studying thick specimens. For example, the 200 CX was designed for low contrast operation at high accelerating voltages up to 200 kV, which offers some advantages for studying thick specimens. The demand for this feature has decreased significantly in recent years and, importantly, the design has compromised operation and low magnification and standard voltages. Moreover, the need for the TEM has increased over the last five years, placing further demands on scheduling and performance of the 200CX. The requested instrument would overcome many of the current problems with the existing TEM and provide key features to improve efficiency and the quality of the images produced. Furthermore, the requested accessories would give the group the ability to perform new types of experiments not currently possible. Many students require the examination of specimens with relatively low inherent contrast, such as specimens with gold-antibody complexes. The patented twin objective lens of the Phillips CM120 is especially designed for examining biological specimens at low magnifications with high contrast and a wide field of view. The high resolution bottom mount CCD digital camera accessory would permit collection of digitized images for on-line Fourier analysis, computerized tomography, automatic montaging, low/minimum dose imaging and production of publication quality prints, thus replacing conventional films for many applications. These and other features are ideally suited for the multi-disciplinary projects requiring TEM. This instrument would greatly enhanced the productivity of the large group of experienced scientists, nine from the Department of Cell Biology & Anatomy and five from other departments at the University, and significantly improve the microscopic facilities in the community.