FIBER CELL JUNCTIONS IN NORMAL AND CATARACTOUS LENSES

描述：纤维细胞的超微结构和功能的持续研究 提出了正常老化和白内障晶状体中的结合点。高 对新鲜晶状体振动组切片的分辨率光镜研究将用于 补充类似制剂的EM研究，允许极好的 两者之间的关联度。其主要目的是使用 比较正常晶状体和白内障晶状体以确定光的原因 散开。中心假说是质膜的破裂 紧随其后的是细胞质内容物的泄漏，造成局部折射 单元格界面的指数变化。还将评估一个)单元格 用共聚焦显微镜观察核芯中的活性b)保存 用透射电子显微镜观察最老的细胞膜，c)胞质的傅里叶分析 质地d)细胞外蛋白质样沉积的定义 太空。原子核中的光散射中心也将被表征。 人类糖尿病晶状体和自发发生的糖尿病犬晶状体。

DESCRIPTION: Continued ultrastructural and functional studies of fiber cell junctions in normal aging and cataractous lenses are proposed. High resolution LM studies on vibratome sections of fresh lenses will be used to complement EM studies on similar preparations, permitting an excellent degree of correlation between the two. The principal aim is to use comparison of normal and cataractous lenses to define the causes of light scatter. The central hypothesis is that the breakdown of plasma membranes is followed by a leakage of cytoplasmic contents creating local refractive index variations at cell interfaces. Also evaluated will be a) cell viability in the nuclear core using confocal microcopy b) the preservation of membranes in the oldest cells by TEM, c) Fourier analysis of cytoplasmic texture d) the definition of protein-like deposits in the extracellular space. Also characterized will be light scatter centers in nuclei from human diabetic lenses, and spontaneously occurring diabetic canine lenses.