UPGRADING CORE FACILITY FOR DNA SEQUENCING AND MAPPING

升级 DNA 测序和绘图的核心设施

• 批准号: 2803498
• 负责人: NARAYAN G AVADHANI
• 金额: $ 13.4万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2803498
• 关键词: DNA; biomedical equipment; biomedical equipment purchase; computer assisted sequence analysis; genetic mapping; genotype; nucleic acid sequence

It is proposed to upgrade the existing DNA core facility by adding an ABI PRISM 377 DNA sequencer, with an upgraded sequence hardware and GENESCAN, GENOTYPER and sequence upgrade softwares. The instrument will be housed and operated in the recently renovated core facility at the School of Veterinary Medicine, and supervised by a Committee of major users from 11 different departments of the School of Veterinary Medicine and School of Medicine, all located within a one to two block area of the campus. The major users consist of 31 research groups (over 170 research personnel) with over 88 federal and non-federal grants, who will use this equipment for about 85% of the time. The research interests of the major user group are focused on the basic as well as clinical aspects of cell and molecular biology, including transgenesis, are focused on the basic as well as clinical aspects of cell and molecular biology, including transgenesis. developmental aspects of histone, hemoglobin, and immunoglobin gene expression, gene expressing during embryogenesis, myogenesis, mitochondrial and nuclear gene interaction, sexual dimorphism in hepatic and brain drug metabolism, teratogenesis, cell transformation, hypoxia/perfusion, structure and function of ion channels, signal transduction in neural cells, AIDS and related neuromuscular and hematopeitic diseases, characterization of genetic disorders of inherited metabolic diseases and development of gene therapy in various animal models. All of the 31 major users have requirements for high efficiency DNA sequencing. About 12 of them have immediate requirements for mutational analysis and gene mapping techniques, and an additional 6 groups need these facilities within the next 2-3 years. The facility will be run by an experienced biochemist/molecular biologist who has been managing the core facility for the past nine years.

建议通过增加ABI PRISM 377 DNA测序仪、升级的测序硬件和GENESCAN、GENOTYPER和序列升级软件来升级现有的DNA核心设施。该仪器将被安置在兽医学院最近翻新的核心设施中，并由来自兽医学院和医学院11个不同部门的主要用户委员会监督，所有这些部门都位于校园的一到两个街区内。主要用户包括31个研究小组（超过170名研究人员），获得了超过88项联邦和非联邦赠款，他们将在大约85%的时间内使用该设备。主要用户组的研究兴趣集中在细胞和分子生物学的基础和临床方面，包括转基因，集中在细胞和分子生物学的基础和临床方面，包括转基因。组蛋白、血红蛋白和免疫球蛋白基因表达的发育方面，胚胎发生过程中的基因表达，肌发生，线粒体和核基因相互作用，肝和脑药物代谢中的性二态性，致畸作用，细胞转化，缺氧/灌注，离子通道的结构和功能，神经细胞中的信号转导，AIDS和相关的神经肌肉和造血疾病，遗传性代谢疾病的遗传性疾病的表征和在各种动物模型中的基因治疗的开发。所有31个主要用户都有高效DNA测序的需求。其中约12个小组急需突变分析和基因绘图技术，另外6个小组在今后2-3年内需要这些设施。该设施将由一位经验丰富的生物化学家/分子生物学家管理，他在过去九年中一直管理核心设施。