PERSEPTIVE BIOSYSTEMS MALDI-TOF MASS SPECTROMETER

透视生物系统 MALDI-TOF 质谱仪

• 批准号: 2791805
• 负责人: GREGORY A GRANT
• 金额: $ 24.96万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2791805
• 关键词: analytical chemistry; biomedical equipment; biomedical equipment purchase; matrix assisted laser desorption ionization

The goal of this application is to enhance the mass spectrometry capabilities available to NIH investigators at Washington University School of Medicine and in so doing significantly strengthen the potential of those investigators to conduct outstanding biomedical research. This will be accomplished by the purchase of a modern Perseptive Biosystems MALDI-TOF mass spectrometer to complement the Finnigan LCQ ESI instrument already in operation. This instrument will enhance the capabilities of NIH supported investigators at Washington University by providing increased sensitivity, versatility of chemistry, and cost-effectiveness in mass spectrometry services. The instrument will be integrated into a Core Facility and be operated by the Washington University Protein and Nucleic Acid Chemistry Laboratories as a shared instrument for biomedical research. A major group of 6 NIH supported investigators has been identified as primary users whose research will be enhanced immediately by acquisition of the instrument. These investigators are funded by 5 different institutes of NIH and require the instrument for projects that include biochemical, biophysical, and structure-function studies in coagulation, the role of inositol phosphates in human disease, enzymatic mechanism of allostery, pili assembly in bacterial pathogenesis, RNA binding function, and gene expression in bone. In addition, the instrument will be utilized by an additional group of approximately 52 Acid Chemistry Laboratories. Finally, approximately 80 additional NIH funded investigators who use the Nucleic Acid Chemistry Laboratories have operated as a core service facility for over 15 years. Thus, all of the necessary requirements for scientific expertise, operation, maintenance, and cost-recovery are in place. In addition, the staff of the Protein and Nucleic Acid Chemistry Laboratories have over 5 years of experience in mass spectrometry analysis.

该应用程序的目标是增强华盛顿大学医学院NIH研究人员可用的质谱学能力，从而显著增强这些研究人员进行杰出生物医学研究的潜力。这将通过购买一台现代渗透生物系统MALDI-TOF质谱仪来实现，以补充已经投入使用的芬兰LCQ ESI仪器。该仪器将通过在质谱学服务中提供更高的灵敏度、化学的多功能性和成本效益来增强华盛顿大学NIH支持的研究人员的能力。该仪器将被整合到一个核心设施中，并由华盛顿大学蛋白质和核酸化学实验室作为生物医学研究的共享仪器进行操作。一个由6名NIH支持的研究人员组成的主要小组已被确定为主要用户，他们的研究将通过获得该仪器而立即得到加强。这些研究人员由NIH的5个不同研究所资助，需要该仪器进行以下项目：凝血中的生化、生物物理和结构-功能研究、肌醇磷酸盐在人类疾病中的作用、变构的酶机制、细菌发病中的菌毛组装、RNA结合功能和骨中的基因表达。此外，该仪器将由另外约52个酸性化学实验室使用。最后，另外约80名由美国国立卫生研究院资助的使用核酸化学实验室的研究人员已作为核心服务设施运营了15年以上。因此，科学专门知识、操作、维护和成本回收方面的所有必要要求都已到位。此外，蛋白质和核酸化学实验室的工作人员在质谱分析方面有5年以上的经验。