BIACORE 2000 SYSTEM

BIACORE 2000 系统

• 批准号: 2767272
• 负责人: ROSALIE K CROUCH
• 金额: $ 23.8万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-19 至 2000-02-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2767272
• 关键词: biomedical equipment; biomedical equipment purchase; surface plasmon resonance

The purpose of this proposal is to obtain a BIACOR 2000 to establish a surface plasmon resonance (SPR) facility at the Medical University of South Carolina (MUSC). The instrument will be shared by five Major Users and two Auxiliary Users in four Departments in the College of Medicine. There are already extensive interactions among these faculty, as they are members of interdepartmental programs in Molecular and Cellular Biology and Pathobiology and Signal Transduction. Four Resource Scientists (two internal, two external) will advise on critical issues of implementation, resource management, data analysis and interpretation, and interface with other biomolecular analytic techniques. Specific aims are: 1) To establish a Surface Plasmon Resonance Facility within the MUSC Biotechnology Resource Laboratory, and to ensure that instrument capability is well supported by data analysis and modeling capabilities; 2) To advance the application of mass spectrometry for analysis of the material bound to the sensor surface; 3) To use this facility to enhance the productivity of NIH- funded investigators and to improve the research efficiency and competitiveness of MUSC research faculty and trainees; 4) To use this facility to further enhance interdisciplinary work among the users. Acquisition of the BIAcore instrument addresses users' critical needs for biosensor technology to identify and characterize a wide range of biomolecular interactions in real-time. Projects to be supported by this instrument include extracellular matrix protein-protein interactions, retinoid protein-ligand interactions, lipid second messenger (ceramide) binding interactions, kinetics and specificity of GTP subunit interactions, Gbetagamma interactions with receptor subdomains, and imidazoline binding domains on MAO B. Auxiliary uses include determining RNA binding affinities and transcription factor interactions. The BIACORE 2000 will greatly augment more traditional thermodynamic and kinetic methods now employed to analyze biomolecular interactions. There is currently at MUSC no other means of analyzing the kinetics of molecular interactions with the accuracy and efficiency of the BIACORE 2000, or with as broad a temperature range. A detailed organizational plan, substantial institutional support, and a long-term plan for maintenance ensure the sustained viability of the facility. Its availability will provide unique opportunities for the development of powerful biophysical capabilities at our institution. It will serve as a platform for interdisciplinary investigation and training, and will significantly enhance the competitiveness of MUSC investigators.

本提案的目的是获得BIACOR 2000，以在南卡罗来纳州医科大学（MUSC）建立表面等离子体共振（SPR）设施。 该仪器将由医学院四个系的五个主要用户和两个辅助用户共享。 这些教师之间已经有了广泛的互动，因为他们是分子和细胞生物学，病理生物学和信号转导跨部门计划的成员。 四名资源科学家（两名内部，两名外部）将就实施，资源管理，数据分析和解释以及与其他生物分子分析技术的接口的关键问题提供建议。 具体目标是：1）在MUSC生物技术资源实验室内建立表面等离子体共振设施，并确保仪器能力得到数据分析和建模能力的良好支持; 2）推进质谱法在分析与传感器表面结合的材料中的应用; 3）利用这一设施来提高NIH资助的研究人员的生产力，并提高MUSC研究人员和学员的研究效率和竞争力; 4）利用这一设施进一步加强用户之间的跨学科工作。BIAcore仪器的收购满足了用户对生物传感器技术的关键需求，以实时识别和表征广泛的生物分子相互作用。 该仪器支持的项目包括细胞外基质蛋白-蛋白相互作用、类维生素A蛋白-配体相互作用、脂质第二信使（神经酰胺）结合相互作用、GTP亚基相互作用的动力学和特异性、Gbetagamma与受体亚结构域的相互作用以及MAO B上的咪唑啉结合结构域。 辅助用途包括确定RNA结合亲和力和转录因子相互作用。 BIACORE 2000将大大增强目前用于分析生物分子相互作用的传统热力学和动力学方法。 目前，在MUSC没有其他方法可以像BIACORE 2000那样准确和高效地分析分子相互作用的动力学，或者在同样宽的温度范围内。 详细的组织计划、大量的机构支持和长期的维护计划确保了该设施的持续生存能力。它的可用性将为我们机构发展强大的生物物理能力提供独特的机会。 它将作为跨学科调查和培训的平台，并将大大提高MUSC调查人员的竞争力。