ANALYTICAL ULTRACENTRIFUGE FACILITY

分析超速离心设备

• 批准号: 2802619
• 负责人: BARBARA M BRODSKY
• 金额: $ 25.26万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-06 至 2000-01-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2802619
• 关键词: analytical ultracentrifugation; biomedical equipment; biomedical equipment purchase; sedimentation equilibrium; sedimentation velocity

The establishment of a shared analytical ultracentrifuge facility would enhance and expand the research programs of a number of NIH supported researchers at UMDNJ-Robert Wood Johnson Medical School. Some of these researchers are biophysically oriented, while others with a molecular biology focus have increasingly complemented their functional studies with structural characterization, supported by the strong structural biology laboratories in our scientific community. The sedimentation studies proposed in this application would provide information on molecular mass, shape, stoichiometry, and intermolecular interactions in systems ranging from model triple-helix and coiled-coil peptides to regulatory proteins, transcription and elongation factor complexes. The interest of these investigators in applying sedimentation methods to their research projects has been driven by two factors. Firstly, the recent advances in analytical ultracentrifuge design and optics, as well as the automation of data acquisition and analysis, has made the method accessible to motivated scientists as well as dedicated experts. Secondly, as a result of efficient expression systems, solid state peptide synthesis, and improved purification methodologies, the quantities of purified molecules and complexes necessary for analytical ultracentrifuges with improved sensitivities have increasingly become available. The results of the proposed studies using sedimentation, with its rigorous thermodynamic basis, will provide information complementary to existing methodologies and can be integrated with the molecular structure data obtained from NMR spectroscopy and x-ray crystallography. In addition, it is the technique of choice for studies on very high molecular weight complexes and for systems where other methods have yielded ambiguous results. The need of these researchers for an analytical ultracentrifuge cannot be met under existing conditions. There is no academic institution in New Jersey that has such instrumentation and collaborations have provided only limited access to sedimentation studies. Several investigators on this application have been involved in productive collaborations with ultracentrifuge experts at national facilities or in private laboratories. But such collaborations are hampered by their long distance nature, the numerous projects ongoing at each center, and the barriers to exploring new areas or following up interesting observations or problems. No user on this application requires more than 15-20 percent of the time on an analytical ultracentrifuge, so that its purchase by any one individual cannot be justified, and yet the presence of this instrument would greatly strengthen the research programs of all users. A shared analytical ultracentrifugation facility would strengthen the research projects of Robert Wood Johnson Medical School researchers by allowing them to utilize this powerful technique.

建立一个共享的分析性超级计算机设施将加强和扩大UMDNJ-罗伯特伍德约翰逊医学院的一些NIH支持的研究人员的研究计划。 这些研究人员中的一些是生物制药导向的，而另一些以分子生物学为重点的研究人员则越来越多地通过结构表征来补充他们的功能研究，这得到了我们科学界强大的结构生物学实验室的支持。 本申请中提出的沉降研究将提供从模型三螺旋和卷曲螺旋肽到调节蛋白、转录和延伸因子复合物的系统中的分子质量、形状、化学计量和分子间相互作用的信息。 这些研究人员将沉积方法应用于其研究项目的兴趣受到两个因素的驱动。 首先，分析超速离心机设计和光学的最新进展，以及数据采集和分析的自动化，使该方法能够被积极进取的科学家和敬业的专家所采用。其次，由于高效的表达系统、固态肽合成和改进的纯化方法，具有改进灵敏度的分析性超电泳所需的纯化分子和复合物的数量越来越多。 建议的研究结果，使用沉淀，其严格的热力学基础，将提供信息补充现有的方法，并可以整合从NMR光谱和X-射线晶体学获得的分子结构数据。此外，它是研究非常高分子量复合物和其他方法产生模糊结果的系统的首选技术。在现有条件下，这些研究人员对分析性超精密仪器的需要是无法满足的。 新泽西没有学术机构拥有此类仪器，合作只能提供有限的沉积研究机会。 这一应用的几位研究人员与国家设施或私人实验室的超浓缩物专家进行了富有成效的合作。 但是，这种合作受到其远距离性质，每个中心正在进行的众多项目以及探索新领域或跟进有趣的观察或问题的障碍的阻碍。 使用这种仪器的用户都不需要花费超过15%-20%的时间来使用分析型超级仪器，因此任何个人购买这种仪器都是不合理的，然而，这种仪器的出现将大大加强所有用户的研究计划。 一个共享的分析超离心设备将加强罗伯特伍德约翰逊医学院研究人员的研究项目，使他们能够利用这种强大的技术。