CORE--CLINICAL RETROVIROLOGY

临床反病毒学核心单位将继续提供病毒学 支持正在进行的流行病学、免疫生物学、 实验致病机制及临床基础分子核心病毒学 艾滋病毒传播和早期感染。特别是，这种支持包括 微量培养、DNA和RNA检测HIV-1的分离和定量 和量化，以及抗逆转录病毒药物敏感性测试， HIV-1的合胞体诱导表型和血清学程序 抗体、p24抗原和HIV-1中和。实验室里有 在开发敏感的艾滋病毒培养技术方面发挥了主导作用 外周血单核细胞和血浆，并已贡献 人类免疫缺陷病毒感染发病机制研究的重要工作 HIV血浆病毒血症的自然病程及定量检测 观察血浆HIV的变化，以评估抗逆转录病毒治疗的疗效。 实验室也有助于我们对脱落的理解 HIV感染者的精液中的HIV。华盛顿大学 病毒学实验室也是世界卫生组织的区域实验室。 1986年--目前执行HIV-1定量检测计划 化验。它已经在各种不同的领域展示了技术专长 病毒学方法，并在ACTG的设计中发挥了重要作用 病毒学方案、计算机化数据管理方案和 质量评估计划，因为其科学的领导力和 技术专长。华盛顿大学逆转录病毒学 实验室是临床核心实验室类型的典范 在这份提案中。

The Clinical Retrovirology Core Unit will continue to provide virologic support to ongoing research on the epidemiologic, immunobiologic, experimental pathogenesis, and basic clinical molecular core virology of HIV transmission and early infection. Specially, this support includes HIV-1 isolation and quantification by microculture, DNA and RNA detection and quantification, and antiretroviral drug sensitivity testing, syncytium-inducing phenotype, and serologic procedures for HIV-1 antibody, p24 antigen, and HIV-1 neutralization. The laboratory has taken a leading role in developing sensitive HIV culture techniques for both peripheral blood mononuclear cells and plasma, and has contributed important work on the pathogenesis of HIV infection by studying the natural history of HIV plasma viremia and the measurement of quantitative changes in plasma HIV to assess the efficacy of antiretroviral therapy. The laboratory has also contributed to our understanding of the shedding of HIV in the semen of HIV-infected men. The University of Washington Virology Laboratory has also served as a regional laboratory for he ACTG program during the years 1986-present for performing quantitative HIV-1 assays. It has demonstrated technical expertise in a wide variety of virologic methods and has been instrumental in the design of the ACTG virology program, the computerized data management program and the quality assessment programs, because of its scientific leadership and technical expertise. The University of Washington Retrovirology Laboratory is a model for the type of clinical core laboratory outlined in this proposal.