CORE--CLINICAL RETROVIROLOGY

临床逆转录病毒学核心单位将继续提供病毒学 支持正在进行的流行病学，免疫生物学， 实验发病机制和基础临床分子核心病毒学 艾滋病毒传播和早期感染。 特别是，这种支持包括 通过微量培养、DNA和RNA检测分离和定量HIV-1 和定量，以及抗逆转录病毒药物敏感性测试， 合胞体诱导表型和HIV-1血清学方法 抗体、p24抗原和HIV-1中和。 实验室已 在开发敏感的艾滋病毒培养技术方面发挥了主导作用， 外周血单核细胞和血浆，并有助于 艾滋病病毒感染的发病机制的重要工作，研究 HIV血浆病毒血症的自然史和定量测定 血浆HIV的变化来评估抗逆转录病毒治疗的疗效。 该实验室也有助于我们了解脱落 HIV感染者精液中的HIV含量。 华盛顿大学 病毒学实验室还担任ACTG的区域实验室 在1986年至今的几年里，该计划用于进行定量HIV-1 测定。 它在各种各样的领域都展示了技术专长， 病毒学方法，并在ACTG的设计中发挥了作用 病毒学程序、计算机化数据管理程序和 质量评估计划，由于其科学的领导和 技术专长。 华盛顿大学逆转录病毒学 实验室是一个模式的类型临床核心实验室概述 在这个提议中。

The Clinical Retrovirology Core Unit will continue to provide virologic support to ongoing research on the epidemiologic, immunobiologic, experimental pathogenesis, and basic clinical molecular core virology of HIV transmission and early infection. Specially, this support includes HIV-1 isolation and quantification by microculture, DNA and RNA detection and quantification, and antiretroviral drug sensitivity testing, syncytium-inducing phenotype, and serologic procedures for HIV-1 antibody, p24 antigen, and HIV-1 neutralization. The laboratory has taken a leading role in developing sensitive HIV culture techniques for both peripheral blood mononuclear cells and plasma, and has contributed important work on the pathogenesis of HIV infection by studying the natural history of HIV plasma viremia and the measurement of quantitative changes in plasma HIV to assess the efficacy of antiretroviral therapy. The laboratory has also contributed to our understanding of the shedding of HIV in the semen of HIV-infected men. The University of Washington Virology Laboratory has also served as a regional laboratory for he ACTG program during the years 1986-present for performing quantitative HIV-1 assays. It has demonstrated technical expertise in a wide variety of virologic methods and has been instrumental in the design of the ACTG virology program, the computerized data management program and the quality assessment programs, because of its scientific leadership and technical expertise. The University of Washington Retrovirology Laboratory is a model for the type of clinical core laboratory outlined in this proposal.