OPTICAL COMPONENT USED FOR MULTIPHOTON MICROSCOPY EFFECTS ON CHAR OF LASER PULSE

本项目的目标是研究的动力学和机制， 不同辐照条件下的NADH光漂白。 大部分 我们的研究中心是活细胞的自发荧光成像 和组织。 由于NADH是由细胞调节的，并且对于 正常的细胞功能，成像过程中的漂白可能会产生深远的影响。 影响细胞的健康。 在初步实验中，我们 测量了低强度单光子漂白截面 辐照 未来的测量将检查和比较一个和两个 光子高强度照明。 由于最初的光漂白 文献中引用的单光子激发机制包括 酶活性NAD的产生，我们将测量和 比较导致酶促还原的漂白的NADH的分数， 不同辐照条件下的活性NAD。

The goal of this project is examine the kinetics and mechanism of NADH photobleaching under different irradiation conditions. Much of our research centers around autofluorescence imaging of living cells and tissue. Since NADH is regulated by the cell and important for proper cell function, bleaching during imaging could have a profound effect on the health of the cell. In the preliminary experiments we measured the one-photon bleaching cross section with low intensity irradiation. Future measurements will examine and compare one and two photon high intensity illumination. Since the primary photobleaching mechanism cited in the literature for one photon excitation involves the generation of enzymatically active NAD, we will measure and compare the fraction of bleached NADH that leads to enzymatically active NAD for the different irradiation conditions..