OPTICAL COMPONENT USED FOR MULTIPHOTON MICROSCOPY EFFECTS ON CHAR OF LASER PULSE

该项目的目标是研究动力学和机制 不同照射条件下NADH光漂白。 很多 我们的研究中心是活细胞的自发荧光成像 和组织。 由于 NADH 受细胞调节并且对于 正常的细胞功能，成像过程中的漂白可能会产生深远的影响 对细胞健康的影响。 在初步实验中我们 测量低强度的单光子漂白截面 辐照。 未来的测量将检查和比较一和二 光子高强度照明。 自初次光漂白以来 文献中引用的单光子激发机制涉及 产生具有酶活性的 NAD，我们将测量并 比较导致酶促反应的漂白 NADH 的分数 不同照射条件下的活性NAD..

The goal of this project is examine the kinetics and mechanism of NADH photobleaching under different irradiation conditions. Much of our research centers around autofluorescence imaging of living cells and tissue. Since NADH is regulated by the cell and important for proper cell function, bleaching during imaging could have a profound effect on the health of the cell. In the preliminary experiments we measured the one-photon bleaching cross section with low intensity irradiation. Future measurements will examine and compare one and two photon high intensity illumination. Since the primary photobleaching mechanism cited in the literature for one photon excitation involves the generation of enzymatically active NAD, we will measure and compare the fraction of bleached NADH that leads to enzymatically active NAD for the different irradiation conditions..