ACTION SPECTRUM OF DNA SYNTHESIS INHIBITION USING FEMTOSECOND ILLUMINATION

我们已经制作了一个光损伤的作用光谱， 飞秒照明从700至900纳米，通过监测的开始， 不添加染料的培养HeLa细胞中的DNA合成抑制。 剂量（Int）定义为在辐照剂量下峰值辐照度的n次幂。 样本乘以焦点体积的总停留时间， cell. (The辐照度，I，单位为光子/s（（m2），n为 单个激发事件所需的光子，或激发 订购。）由于我们尚未确定的激发顺序 在所有波长处的损伤，该作用光谱表示为n = 2、定义双光子事件。 测得的损伤阈值为 高于典型成像剂量的数量级。

We have produced an action spectrum of photodamage using femtosecond illumination from 700 to 900 nm by monitoring the onset of DNA synthesis inhibition in cultured HeLa cells without added dyes. Doses (Int) are defined as the nth power of the peak irradiance at the sample multiplied by the total dwell time of the focal volume in a cell. (The irradiance, I, is in photons/s((m2, and n is the number of photons required for a single excitation event, or the excitation order.) Since we have not yet determined the excitation order of damage at all wavelengths, this action spectrum is expressed with n = 2, defining two-photon events. The measured damage thresholds are orders of magnitude above typical imaging doses.