IMMUNOLOCALIZATION OF CONNEXIN 50 IN HYPOTHALAMUS

下丘脑中连接蛋白 50 的免疫定位

• 批准号: 6220685
• 负责人: FREDERIC MERCIER
• 金额: $ 1.92万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-15 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6220685
• 关键词: Mammalia; aging; biomedical resource; microscopy; nervous system; substance abuse related disorder; technology /technique

Astrocytes are thought to play an important role in regulating the composition of the extracellular environment in the nervous system. Both anatomical and physiological investigations have localized various ion channels and neurotransmitter uptake systems to astrocytic processes within the neuropil. Computer modeling offers one avenue for investigating the capacity of astrocytic processes to affect the extracellular milieu surrounding neuronal cell bodies, axons and dendrites. In order to construct such models, accurate measurements of surface area and volume of individual astrocytes will be necessary. Golgi-impregnation of astrocytes indicates that each astrocyte possesses an extensive array of fine processes which greatly increase surface area but which cannot be resolved by the light microscope. In the present collaboration, we are using tomographic reconstructions to obtain surface area and volume measurements of Golgi-impregnated astrocytes. In some cases, entire ast rocytes will be reconstructed using serial tomography. This work is being carried out jointly at the NCMIR using the IVEM and at the National Institute for Physiological Sciences in Okasaki, Japan, using the 1meV electron microscope. We have been participating in a scientific exchange program in order to implement the routines for performing tomographic reconstructions developed at NCMIR in Okasaki. Currently, all of the software has been implemented.

星形胶质细胞被认为在调节大脑的神经活动中起着重要作用