IMMUNOLOCALIZATION OF CONNEXIN 50 IN HYPOTHALAMUS

星形胶质细胞被认为在调节 神经系统中细胞外环境的组成。 解剖学和生理学研究都局限于 星形胶质细胞的多种离子通道和神经递质摄取系统 神经纤维束内的突起。计算机建模提供了一种途径 为了研究星形细胞突起影响 神经元胞体、轴突和周围的细胞外环境 树枝状结构。为了构建这样的模型，准确的测量 单个星形胶质细胞的表面积和体积将是必要的。 星形胶质细胞高尔基体染色表明每个星形胶质细胞 拥有广泛的精细工艺，大大增加了 光学显微镜无法分辨的表面积。在……里面 在目前的合作中，我们正在使用断层重建来 测得浸渍高尔基体的表面积和体积 星形胶质细胞。在某些情况下，整个胚胎细胞将被重建。 使用连续断层扫描。这项工作正在联合在 使用IVEM的NCMIR和在美国国立卫生研究院 使用1 MeV电子的日本冈崎的生理科学 显微镜。我们一直在参加一次科学交流 程序，以实现用于执行断层扫描的例程 在冈崎的NCMIR进行了重建。目前，所有的 软件已经实现。

Astrocytes are thought to play an important role in regulating the composition of the extracellular environment in the nervous system. Both anatomical and physiological investigations have localized various ion channels and neurotransmitter uptake systems to astrocytic processes within the neuropil. Computer modeling offers one avenue for investigating the capacity of astrocytic processes to affect the extracellular milieu surrounding neuronal cell bodies, axons and dendrites. In order to construct such models, accurate measurements of surface area and volume of individual astrocytes will be necessary. Golgi-impregnation of astrocytes indicates that each astrocyte possesses an extensive array of fine processes which greatly increase surface area but which cannot be resolved by the light microscope. In the present collaboration, we are using tomographic reconstructions to obtain surface area and volume measurements of Golgi-impregnated astrocytes. In some cases, entire ast rocytes will be reconstructed using serial tomography. This work is being carried out jointly at the NCMIR using the IVEM and at the National Institute for Physiological Sciences in Okasaki, Japan, using the 1meV electron microscope. We have been participating in a scientific exchange program in order to implement the routines for performing tomographic reconstructions developed at NCMIR in Okasaki. Currently, all of the software has been implemented.