IMMUNOLOCALIZATION OF CONNEXIN 50 IN HYPOTHALAMUS

星形胶质细胞被认为在调节细胞增殖中起重要作用。 神经系统中细胞外环境的组成。 解剖学和生理学研究都定位了 各种离子通道和神经递质摄取系统， 神经元内的过程。 计算机建模提供了一种途径 用于研究星形胶质细胞过程的能力， 神经元细胞体、轴突和周围的细胞外环境 树突 为了构建这样的模型，精确的测量 单个星形胶质细胞的表面积和体积将是必要的。 星形胶质细胞的高尔基体浸渍表明每个星形胶质细胞 拥有广泛的精细工艺，大大提高了 表面积，但不能解决的光学显微镜。 在 目前的合作，我们正在使用断层重建， 获得高尔基体浸渍的表面积和体积测量值 星形胶质细胞 在某些情况下，整个星形细胞将被重建， 使用连续断层扫描。 这项工作正在联合进行， 使用IVEM的NCMR和国家研究所 日本冈崎的生理科学，使用1meV电子 显微镜 我们参加了一个科学交流会 程序，以便执行用于执行断层扫描的例程 在冈崎的NCMIR开发的重建。 目前，所有 软件已经实现。

Astrocytes are thought to play an important role in regulating the composition of the extracellular environment in the nervous system. Both anatomical and physiological investigations have localized various ion channels and neurotransmitter uptake systems to astrocytic processes within the neuropil. Computer modeling offers one avenue for investigating the capacity of astrocytic processes to affect the extracellular milieu surrounding neuronal cell bodies, axons and dendrites. In order to construct such models, accurate measurements of surface area and volume of individual astrocytes will be necessary. Golgi-impregnation of astrocytes indicates that each astrocyte possesses an extensive array of fine processes which greatly increase surface area but which cannot be resolved by the light microscope. In the present collaboration, we are using tomographic reconstructions to obtain surface area and volume measurements of Golgi-impregnated astrocytes. In some cases, entire ast rocytes will be reconstructed using serial tomography. This work is being carried out jointly at the NCMIR using the IVEM and at the National Institute for Physiological Sciences in Okasaki, Japan, using the 1meV electron microscope. We have been participating in a scientific exchange program in order to implement the routines for performing tomographic reconstructions developed at NCMIR in Okasaki. Currently, all of the software has been implemented.