CYTOSKELETAL TARGETS OF NEURONAL SIGNALING

我们已经开发了单克隆抗体， 自身抗体，结合氧化LDL，特别是氧化LDL， 磷脂 这些表位存在于氧化低密度脂蛋白的脂质和 和蛋白质相（后者作为氧化磷脂的缀合物 蛋白质）。到目前为止，我们的分析表明，这些结合不仅 氧化低密度脂蛋白，而且凋亡细胞（其经历增强的氧化 stress.）我们使用共聚焦显微镜来帮助记录 这些单克隆抗体的定位和细胞结合。 因此 到目前为止，我们已经确定这些表位存在于细胞上， 凋亡细胞膜。

We have developed monoclonal antibodies, both induced and autoantibodies, that bind to oxidized LDL and specifically to oxidized phospholipids. These epitopes are present on OxLDL in both the lipid and protein phase (the latter as conjugates of oxidized phospholipids to protein.) By far analysis we have shown these bind not only to OxLDL but also to apoptotic cells (which undergo enhanced oxidative stress.) We are using confocal microscopy to help document the location and cellular binding of these monoclonal antibodies. Thus far, we have determined that these epitopes are present on the cell membrane of apoptotic cells.