PROTEINS AND DNA--NEW METHODS OF ADDUCT DETECTION

描述：本部分研究计划的总体目标是 将分子方法应用于生物标志物的开发， 暴露于人类环境中的一些关键有毒成分 生活在阿伯乔纳地区这些生物标志物将容易地包括 血液中的蛋白质和DNA 和潜在目标器官细胞的DNA拟议研究 是他们早期研究的延续和扩展 in these same相同areas地区.这些方法将包括最先进的 质谱法，低温荧光光谱法， 和修饰的DNA的后标记分析。污染物会 研究的主要焦点将是多环芳烃 碳氢化合物（PAH），代表从空气和食物中的暴露，以及砷 和铬，代表暴露于水。通过检查 血清白蛋白和组蛋白以及DNA，他们将尝试开发这两种蛋白， 暴露和损害的短期和长期尺度。综合用途 35S后标记和激光荧光和电喷雾质量 光谱法将为生物样品的分析提供无与伦比的灵敏度和特异性。 生物标志物在流行病学中的应用将添加单克隆抗体 对那些已经做出的氧化反应进行快速准确的分析， 修改DNA将进行实验以测试 铬和砷的相互作用。最后，所有开发 生物标志物将被用于对人类的暴露研究， 阿伯乔纳地区。

Description: The overall goals of this part of the research program are to apply molecular approaches to the development of biomarkers of exposure to some key toxic components in the environment of people living in the Aberjona region. These biomarkers will include readily accessible blood proteins and DNA from cells in blood and also protein and DNA from cells in potential target organs. The proposed research is a continuation and amplification of their earlier ongoing research in these same areas. The methods will include the most advanced approaches in mass spectrometry, cryogenic fluorescence spectroscopy, and postlabeling analysis of modified DNA. The contaminants that will be the main focus of the research will be the polycyclic aromatic hydrocarbons (PAH), representing exposure from air and food, and arsenic and chromium, representing exposure from water. Through examination of both serum albumin and histones and DNA they will try to develop both a short- and long- term scale for exposure and damage. The combined uses of 35S postlabeling and laser fluorescence and electrospray mass spectrometry will give unparalleled sensitivity and specificity to the use of biomarkers in epidemiology. Monoclonal antibodies will be added to those already made to give rapid and accurate analysis of oxidatively modified DNA. Experiments will be conducted to test the possibility of interactions between chromium and arsenic. Finally, all developed biomarkers will be employed in exposure studies on people in the Aberjona region.