PROTEINS AND DNA--NEW METHODS OF ADDUCT DETECTION

描述：这部分研究计划的总体目标是 将分子方法应用于生物标记物的开发 暴露于人类环境中的一些关键有毒成分 居住在阿伯乔纳地区。这些生物标志物将很容易包括 可获得的血液蛋白质和来自血液细胞的DNA以及蛋白质 以及来自潜在靶器官细胞的DNA。拟议的研究 是对他们早期正在进行的研究的延续和放大 在这些相同的区域。这些方法将包括最先进的 质谱学、低温荧光光谱学 对修饰的DNA进行后标记分析。污染物将会 多环芳香族化合物将成为研究的重点 碳氢化合物(PAH)，代表从空气和食物中暴露，以及砷 和铬，代表暴露在水中。通过对…的审查 包括血清白蛋白、组蛋白和DNA，他们将尝试发展这两者 暴露和损害的短期和长期规模。组合使用 ~(35)S后标记和激光荧光及电喷雾质量 光谱分析将给出无与伦比的灵敏度和特异性 生物标志物在流行病学中的应用。将添加单抗 对于那些已经做出的快速准确的氧化性分析 修改过的DNA。将进行实验，以测试 铬和砷之间的相互作用。最后，所有人都发展起来了 生物标记物将被用于对中国人的暴露研究 阿伯乔纳地区。

Description: The overall goals of this part of the research program are to apply molecular approaches to the development of biomarkers of exposure to some key toxic components in the environment of people living in the Aberjona region. These biomarkers will include readily accessible blood proteins and DNA from cells in blood and also protein and DNA from cells in potential target organs. The proposed research is a continuation and amplification of their earlier ongoing research in these same areas. The methods will include the most advanced approaches in mass spectrometry, cryogenic fluorescence spectroscopy, and postlabeling analysis of modified DNA. The contaminants that will be the main focus of the research will be the polycyclic aromatic hydrocarbons (PAH), representing exposure from air and food, and arsenic and chromium, representing exposure from water. Through examination of both serum albumin and histones and DNA they will try to develop both a short- and long- term scale for exposure and damage. The combined uses of 35S postlabeling and laser fluorescence and electrospray mass spectrometry will give unparalleled sensitivity and specificity to the use of biomarkers in epidemiology. Monoclonal antibodies will be added to those already made to give rapid and accurate analysis of oxidatively modified DNA. Experiments will be conducted to test the possibility of interactions between chromium and arsenic. Finally, all developed biomarkers will be employed in exposure studies on people in the Aberjona region.