GENETIC ANALYSIS OF SEPTIN FUNCTION IN FLIES AND YEAST

果蝇和酵母中 Septin 功能的遗传分析

• 批准号: 6294514
• 负责人: KAREN G HALES
• 金额: $ 1.04万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6294514
• 关键词: Drosophilidae; Saccharomyces cerevisiae; cell cycle; cytogenetics; cytoskeletal proteins; gene mutation; genetic mapping; genetically modified animals; green fluorescent proteins; immunofluorescence technique; protein localization; protein structure function; site directed mutagenesis; western blottings

The proposed research aims to elucidate the basic general function of septin proteins in multicellular organisms, specifically in Drosophila melanogaster. A role for septins, which are multidomain GTPases that form filaments, is established in yeast cytokinesis; however there are hints that septins have more general roles in directed membrane insertion and organization of the cell cortex. Through generation and examination of mutant Drosophila strains defective in particular septins, a broader definition of septin function in different cell types will be established. A second goal of the proposed research is to dissect the biological relationship between septins and conjugation to ubiquitin-like proteins of the Smt3p family. Genetic manipulation in the yeast Saccharomyces cerevisiae will distinguish whether septin subcellular localization depends upon conjugation to an Smt3p moiety, or whether septins modulate Smt3p conjugation to other proteins. One molecular mechanism by which septins participate in cortical organization will thus be elucidated.

拟议的研究旨在阐明多细胞生物（特别是黑腹果蝇）中脓毒蛋白的基本功能。 脓蛋白（一种形成细丝的多域 GTP 酶）在酵母胞质分裂中发挥作用。然而，有迹象表明脓毒症在细胞皮层的定向膜插入和组织中具有更普遍的作用。 通过产生和检查特定败血症缺陷的突变果蝇菌株，将建立不同细胞类型中败血症功能的更广泛的定义。该研究的第二个目标是剖析脓毒症与 Smt3p 家族泛素样蛋白缀合之间的生物学关系。 酿酒酵母中的基因操作将区分 septin 亚细胞定位是否依赖于与 Smt3p 部分的缀合，或者 septin 是否调节 Smt3p 与其他蛋白质的缀合。 因此，将阐明脓毒症参与皮质组织的一种分子机制。