T CELL TURNOVER IN HIV INFECTED CHILDREN AND ADOLESCENTS

HIV 感染儿童和青少年的 T 细胞更新

• 批准号: 2889588
• 负责人: Stuart E Starr
• 金额: $ 26.25万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2889588
• 关键词: CD antigens; CD4 molecule; CD8 molecule; HIV infections; T lymphocyte; adolescence (12-20); cell population study; child (0-11); clinical research; flow cytometry; human subject; leukocyte activation /transformation; medical complication; phosphoprotein phosphatase; stable isotope; thymus

The applicants plan to evaluate the turnover of naive (CD45 RA) and memory (CD45RO) CD4 and CD8 lymphocytes in HIV-infected children and adolescents using several methods, specifically: DNA labeling with stable-isotope labeled glucose; telomere length; and, cell cycle/apoptosis by flow cytometry. Data will be obtained before and after 20 and 48 weeks of highly active antiretroviral therapy. Age- matched uninfected children will be tested for comparison. To define better the role of the thymus in turnover of naive and memory CD4 and CD8 cells, children who as infants underwent thymectomy during cardiac surgery and age-matched healthy children will be studied using the same methods as those used for HIV-infected children. The applicant states that these studies will provide information on 1) the role of the thymus in T cell lymphocyte homeostasis at different ages, 2) the effect of HIV infection on cell turnover and, 3) the effect of HAART on T cell turnover in HIV-infected children and adolescents.

申请人计划评估初始（CD 45 RA）和 HIV感染儿童的记忆（CD 45 RO）CD 4和CD 8淋巴细胞， 青少年使用几种方法，特别是：DNA标记与 稳定同位素标记的葡萄糖;端粒长度;和，细胞 通过流式细胞术检测细胞周期/凋亡。 数据将在 经过20周和48周的高效抗逆转录病毒治疗。 年龄- 将对匹配的未受感染儿童进行测试以进行比较。 以限定 改善胸腺在幼稚和记忆CD 4周转中的作用， CD 8细胞，婴儿时期接受胸腺切除术的儿童， 手术和年龄匹配的健康儿童将使用相同的 与用于艾滋病毒感染儿童的方法相同。 申请人表示 这些研究将提供以下信息：1）胸腺的作用 在不同年龄的T细胞淋巴细胞稳态，2）艾滋病毒的影响 感染对细胞更新的影响; 3）HAART对T细胞的影响 艾滋病毒感染儿童和青少年的更替率。