T CELL TURNOVER IN HIV INFECTED CHILDREN AND ADOLESCENTS

申请者计划评估NAIVE(CD45 RA)和 HIV感染儿童的记忆(CD45RO)CD4和CD8淋巴细胞及 青少年使用几种方法，特别是：DNA标记 稳定同位素标记的葡萄糖；端粒长度；以及细胞 流式细胞术检测细胞周期/细胞凋亡。数据将在和之前获得 在20周和48周的高效抗逆转录病毒治疗后。年龄- 将对匹配的未感染儿童进行测试，以进行比较。要定义 更好地发挥胸腺在幼稚和记忆中的作用 CD8细胞，婴儿时期在心脏手术中接受胸腺切除术的儿童 手术和年龄匹配的健康儿童将使用相同的研究 方法与用于HIV感染儿童的方法相同。申请者陈述 这些研究将提供关于1)胸腺的作用的信息 不同年龄段T细胞淋巴细胞的动态平衡，2)HIV的影响 感染对细胞周转的影响；3)HAART对T细胞的影响 感染艾滋病毒的儿童和青少年的营业额。

The applicants plan to evaluate the turnover of naive (CD45 RA) and memory (CD45RO) CD4 and CD8 lymphocytes in HIV-infected children and adolescents using several methods, specifically: DNA labeling with stable-isotope labeled glucose; telomere length; and, cell cycle/apoptosis by flow cytometry. Data will be obtained before and after 20 and 48 weeks of highly active antiretroviral therapy. Age- matched uninfected children will be tested for comparison. To define better the role of the thymus in turnover of naive and memory CD4 and CD8 cells, children who as infants underwent thymectomy during cardiac surgery and age-matched healthy children will be studied using the same methods as those used for HIV-infected children. The applicant states that these studies will provide information on 1) the role of the thymus in T cell lymphocyte homeostasis at different ages, 2) the effect of HIV infection on cell turnover and, 3) the effect of HAART on T cell turnover in HIV-infected children and adolescents.