DOPAMINERGIC & GABAERGIC ACTIONS ON PALLIDAL DISCHARGE

多巴胺能

• 批准号: 6219621
• 负责人: MARJORIE E ANDERSON
• 金额: $ 5.72万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6219621
• 关键词: Mammalia; cognition; musculoskeletal system; nervous system; rehabilitation

Our current objectives are to determine the actions of dopamine via D1 and D2 receptors on tonic and phasic discharge of neurons in external and internal pallidal segments, and to study the effects of striatal GABA on striatal output neurons. Pharmacological agents have been applied to the striatum through implanted PAN-PVC tubing in three monkeys. In all, the technique developed by Dr. Dubach has resulted in accurate placement of the tubing along a curved longitudinal trajectory in the lateral portion of the putamen. We have applied the D1-selective antagonist SCH23390 and the D2-selective antagonist raclopide successfully while monitoring behavior and recording from pallidal neurons. In high concentrations similar to those injected by others into the cortex, both agents interrupted behavior, and SCH23390 caused drowsiness. Even at concentrations reduced by 10- to 100-fold, the animal stopped working, but only after a longer drug perfusion and with a shorter wash out time to behavioral recovery. The behavioral data showed that, even when the intervals between successful trials were very long, the reaction time and movement time for successful trials was not significantly longer than the times before or after drug application. This suggests that drug administration alters attention, more than processes directly related to motor output. FUNDING NIH grants RR00166 and NS15017. Anderson, M.E., Dubach, M., Ruffo, M., Eaton, R., and Buford, J.A. Effects on motor behavior of dopamine antagonists applied locally in the putamen of awake monkeys. Soc. Neurosci. Abstr. 24:1650, 1998.

我们目前的目标是确定多巴胺的作用 通过D_1和D_2受体对大脑皮层神经元强直相放电的影响 苍白球的外部和内部节段，并研究其对 纹状体GABA对纹状体输出神经元的影响。药理药剂有 通过植入PAN-PVC管应用于纹状体内 猴子。总而言之，杜巴赫博士开发的技术已经导致 在沿弯曲的纵向准确放置油管时 弹道在壳核的外侧部分。我们已经应用了 D_1选择性拮抗剂SCH23390和D_2选择性拮抗剂 在监控行为和记录时成功使用raclopide 苍白球神经元。在高浓度的情况下，与 其他人进入大脑皮层，两个代理中断行为，和SCH23390 导致昏昏欲睡。即使浓度降低了10到100倍， 动物停止工作，但只是在较长时间的药物灌流和 用更短的时间来恢复行为。行为上的 数据显示，即使成功试验之间的间隔 都很长，反应时间和动作时间都很成功 试验时间并不比试验前或试验后显著延长 药物申请。这表明，药物管理改变了 注意，这不仅仅是与电机输出直接相关的过程。 资助NIH拨款RR00166和NS15017。安德森，M.E.，杜巴赫，M. Ruffo，M.，Eaton，R.和Buford，J.A.对运动行为的影响 多巴胺拮抗剂局部应用于清醒猴子的壳核。 SoC。神经科。节选。24：1650,1998。