DOPAMINERGIC & GABAERGIC ACTIONS ON PALLIDAL DISCHARGE

我们目前的目标是确定多巴胺的作用 通过D1和D2受体对神经元的紧张性和相性放电的影响， 苍白球内外节段，并研究 纹状体GABA对纹状体输出神经元的影响。 药物制剂具有 通过植入的PAN-PVC管应用于纹状体， 猴子 总的来说，Dubach博士开发的技术 在管道沿着弯曲的纵向管道的精确放置中 在壳核的侧部留下了轨迹 我们已经应用了 D1选择性拮抗剂SCH 23390和D2选择性拮抗剂 瑞氯匹德成功，同时监测行为和记录 苍白球神经元 在高浓度下，类似于 其他进入皮层，两种代理中断行为，和SCH 23390 引起困倦。 即使浓度降低10- 100倍， 动物停止工作，但只有在更长的药物灌注后， 行为恢复的洗脱时间更短。 行为 数据显示，即使成功试验的间隔时间 反应时间和动作时间都很长， 试验的时间并没有明显长于试验前或试验后的时间。 药物应用。 这表明药物管理改变了 注意力，多与运动输出直接相关的过程。 资助NIH赠款RR 00166和NS 15017。 安德森，医学博士，Dubach，M.， Ruffo，M.，伊顿河，和Buford，J.A.对运动行为的影响 局部应用于清醒猴子壳核的多巴胺拮抗剂。 Soc. Neurosci. 24：1650，1998.

Our current objectives are to determine the actions of dopamine via D1 and D2 receptors on tonic and phasic discharge of neurons in external and internal pallidal segments, and to study the effects of striatal GABA on striatal output neurons. Pharmacological agents have been applied to the striatum through implanted PAN-PVC tubing in three monkeys. In all, the technique developed by Dr. Dubach has resulted in accurate placement of the tubing along a curved longitudinal trajectory in the lateral portion of the putamen. We have applied the D1-selective antagonist SCH23390 and the D2-selective antagonist raclopide successfully while monitoring behavior and recording from pallidal neurons. In high concentrations similar to those injected by others into the cortex, both agents interrupted behavior, and SCH23390 caused drowsiness. Even at concentrations reduced by 10- to 100-fold, the animal stopped working, but only after a longer drug perfusion and with a shorter wash out time to behavioral recovery. The behavioral data showed that, even when the intervals between successful trials were very long, the reaction time and movement time for successful trials was not significantly longer than the times before or after drug application. This suggests that drug administration alters attention, more than processes directly related to motor output. FUNDING NIH grants RR00166 and NS15017. Anderson, M.E., Dubach, M., Ruffo, M., Eaton, R., and Buford, J.A. Effects on motor behavior of dopamine antagonists applied locally in the putamen of awake monkeys. Soc. Neurosci. Abstr. 24:1650, 1998.