TITRATION OF UNCLONED SIVPBJ14 VIRUS STOCK

已经鉴定了许多SIV分离株，并且已经鉴定了SIVPBj14。 在体外和体内都进行了充分的表征和研究。 这 通常认为特定的病毒分离物具有更高的致病性 与其他菌株如SIVmne和SIVmac251相比。 由于其 SIVPBj14具有致病性，可能适合抗逆转录病毒研究 在猕猴中，因为药物治疗的效果可以在 病毒学和发病机理。 由于这些原因，我们生产了一种无细胞的 用于接种和抗逆转录病毒的未克隆SIVPBj14病毒储备液 猕猴模型的研究。 将该病毒储备液滴定至 猕猴，以确定100%感染剂量。 进一步滴定 将是不必要的，所有未来的抗逆转录病毒药物研究将 对猕猴使用相同的病毒接种物。 1998年，八只猕猴 接种SIVPBj14的小鼠在长期随访研究中保持不变。 资助NIH合同AI65311和NIH赠款RR00166。

Many isolates of SIV have been identified and SIVPBj14 has been well characterized and studied both in vitro and in vivo. This particular viral isolate is generally considered to be more pathogenic than other strains such as SIVmne and SIVmac251. Because of its pathogenic nature, SIVPBj14 may be suitable for antiretroviral studies in macaques since the effects of drug therapy can be monitored on both virology and pathogenesis. For these reasons, we produced a cell-free uncloned SIVPBj14 virus stock for inoculations and antiretroviral studies in the macaque model. This virus stock was titrated in macaques to determine the 100% infectious dose. Further titrations will be unnecessary, and all future antiretroviral drug studies will use the same viral inoculum for the macaques. In 1998 eight macaques inoculated with SIVPBj14 remained in a long-term follow-up study. FUNDING NIH contract AI65311 and NIH grant RR00166.