CORE--TRANSGENIC ANIMAL FACILITY

核心——转基因动物设施

• 批准号: 6217228
• 负责人: ANNE E GRIEP
• 金额: $ 28.96万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-16 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6217228
• 关键词: animal colony; biomedical facility; cryopreservation; disease /disorder model; gene targeting; genetically modified animals; laboratory mouse; laboratory rat; mammalian embryology

The Transgenic Animal Facility provides UW Cancer Center staff with the capacity to manipulate the genome of experimental animals used in cancer research. The efficient generation of knockout or transgenic animals requires specialized technical skills in the areas of animal husbandry and surgery, embryology, embryonic stem culture and gene targeting vectorology, embryo micro-manipulation, and cryopreservation. In addition, the procedures require sensitive and expensive microscopic equipment as well as SPF-animal facilities. Because these requirement cannot be met by most individual investigators in their own laboratories, the gene targeting and transgenic animal technologies must be provided by common resource facilities. At the University of Wisconsin, that resource is the Transgenic Animal Facility (TAF). The cancer research performed at UWCCC relies heavily upon this resource. For that reason, funds are requested to support this critical activity.

转基因动物设施为华盛顿大学癌症中心的工作人员提供 操纵癌症实验动物基因组的能力 research. 基因敲除或转基因动物的有效产生 需要畜牧业领域的专业技术技能， 手术、胚胎学、胚胎干培养和基因靶向 载体学、胚胎显微操作和冷冻保存。此外，本发明还提供了一种方法， 该方法需要敏感且昂贵的显微镜设备， 以及SPF动物设施。因为这些要求不能通过 大多数个体研究者在他们自己的实验室里， 靶向和转基因动物技术必须由共同的 资源设施。在威斯康星州大学，这种资源是 转基因动物设施（TAF）。在UWCCC进行的癌症研究 严重依赖于这种资源。为此，请求提供资金， 支持这一重要活动。