CORE--TRANSGENIC ANIMAL FACILITY

核心——转基因动物设施

• 批准号: 6316511
• 负责人: ANNE E GRIEP
• 金额: $ 28.96万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-30 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6316511
• 关键词: animal colony; biomedical facility; cryopreservation; disease /disorder model; gene targeting; genetically modified animals; laboratory mouse; laboratory rat; mammalian embryology

The Transgenic Animal Facility provides UW Cancer Center staff with the capacity to manipulate the genome of experimental animals used in cancer research. The efficient generation of knockout or transgenic animals requires specialized technical skills in the areas of animal husbandry and surgery, embryology, embryonic stem culture and gene targeting vectorology, embryo micro-manipulation, and cryopreservation. In addition, the procedures require sensitive and expensive microscopic equipment as well as SPF-animal facilities. Because these requirement cannot be met by most individual investigators in their own laboratories, the gene targeting and transgenic animal technologies must be provided by common resource facilities. At the University of Wisconsin, that resource is the Transgenic Animal Facility (TAF). The cancer research performed at UWCCC relies heavily upon this resource. For that reason, funds are requested to support this critical activity.

转基因动物设施为威斯康星大学癌症中心的工作人员提供了