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Transgenic and Mutant Animals

转基因和突变动物

基本信息

批准号：
8250418
负责人：
ANNE E GRIEP
金额：
$ 12.18万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-04-01 至 2013-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8250418
关键词：
Animal Husbandry Animals Biotechnology Breeding Communities Comprehensive Cancer Center Consult Cryopreservation DNA Embryo Engineering Equation Equipment Experimental Designs Female Generations Genomics Human Resources Injection of therapeutic agent Knockout Mice Laboratories Malignant Neoplasms Manuals Microinjections Micromanipulation Microscopic Mission Molecular Biology Molecular Genetics Mus Mutate Nature Ovarian Partner in relationship Preparation Prevention Procedures Process Production Protocols documentation Rattus Reproductive Biology Research Resources Services Southern Blotting Tail Techniques Technology Time Training Transgenic Animals Transgenic Mice Transgenic Organisms Transplantation Universities University of Wisconsin Comprehensive Cancer Center Wisconsin animal breeding animal facility blastocyst embryo surgery embryonic stem cell experience in vivo Model insight investigator training member mutant reproductive research study skills success treatment strategy vector

项目摘要

Transgenic and genetically-engineered mutant animals provide one of the most valuable mechanisms for generating in vivo models for unraveling the molecular and genetic mechanisms of cancer and for evaluating new strategies for treatment and prevention studies. Efficient generation of transgenic animals requires great technical skill that is acquired only after extensive experience with animal husbandry and breeding, animal surgery, embryo manipulation and micromanipulation. The transgenic Animal Shared Service makes transgenic technology accessible and affordable to the members.of the University of Wisconsin Comprehensive Cancer Center. It uses the personnel, facilities and equipment of the campus-wide University of Wisconsin Biotechnology Center's Transgenic Animal Facility. Services currently provided by the Transgenic Animals Core include: Generation of transgenic mice and rats by pronuclear microinjection Cryopreservation of mouse and rat embryos ¿ Generation of knockout mice: generation of targeting vectors, generation of targeted embryonic stem (ES) cell clones, and generation of chimeric mice by injection of ES cells into host blastocyst Strain rederivations and ovarian transplants Other miscellaneous molecular biology and embryo services related to transgenic and knockout mouse production Consulting and training In addition, the staff are available for consulting at any point in the process of using the services. Considerable time is spent discussing experimental design and providing guidance on processes that they themselves are or will "be carrying out. They also train investigators in techniques such as superovulating, mating, and collecting reproductive tracts from female mice for embryo cryopreservation, isolating DMA for pronuclear micronjections, mouse handling and breeding protocols, and cutting tails and genomic DNA preparation Southern blotting to identify correctly mutated ES cells.

转基因和基因工程突变动物提供了一个最有价值的机制，产生体内模型，用于阐明癌症的分子和遗传机制，治疗和预防研究的新策略。转基因动物的高效生产需要大量的在畜牧业和养殖业、动物养殖业手术、胚胎操作和显微操作。转基因动物共享服务使威斯康星州大学的成员可以获得和负担得起的转基因技术综合癌症中心。它利用整个校园的人员、设施和设备，威斯康星州大学生物技术中心的转基因动物设施。目前提供的服务转基因动物核心包括：原核显微注射法制备转基因小鼠和大鼠小鼠和大鼠胚胎的冷冻保存基因敲除小鼠的产生：靶向载体的产生，靶向胚胎干（ES）的产生细胞克隆，以及通过将ES细胞注射到宿主胚泡中产生嵌合小鼠品系再衍生和卵巢移植其他与转基因和基因敲除小鼠相关的其他分子生物学和胚胎服务生产咨询和培训此外，工作人员在使用服务过程中的任何时候都可以咨询。相当多的时间花在讨论实验设计和提供指导的过程中，他们 “他们正在或将要进行。他们还培训调查人员的技术，如超排卵，交配，并从雌性小鼠收集生殖道用于胚胎冷冻保存，分离DMA用于原核微注射，小鼠处理和繁殖方案，剪尾和基因组DNA 制备Southern印迹以鉴定正确突变的ES细胞。