DEREGULATION OF CELL GROWTH BY K1 GENE OF KAPOSIS SARCOMA ASSOCIATED HERPESVIRUS

位于与编码三文鱼的基因相同的位置 卡波西氏疱疹病毒转化蛋白(STP) 肉瘤相关疱疹病毒(KSHV)含有明显的开放读数 名为K1的框架，尽管KSHV和HV是 狂犬病病毒伽马疱疹病毒亚群、K1和STP表现为NO 在氨基酸序列或结构组织上的相似性 STP是人类乳头状瘤致癌潜能所必需的，我们研究了 K1基因K1表达的功能后果 啮齿动物成纤维细胞的形态改变和病灶形成 一种重组疱疹病毒，在这种病毒中 人类疱疹病毒STP癌基因被K1取代，永生化原代T 淋巴细胞对IL-2非依赖性生长和诱导淋巴瘤的共同作用 绒猴这些结果证明了 KSHV的K1基因发表Lee，H，Veazey，R，Williams，K，Li，M， 郭美美，内佩尔，F，弗莱肯斯坦，B，拉克纳，A，Desrosiers，RC和 Kaposi‘s K1基因对细胞生长的解除调控 肉瘤相关疱疹病毒自然医学1998；4：435-440

At a position equivalent to the gene encoding the saimiri transforming protein (STP) of herpesvirus saimiri (HVS), Kaposi's sarcoma-associated herpesvirus (KSHV) contains a distinct open reading frame called K1 Although KSHVand HVS are related members of the rhadinovirus subgroup of gamma herpesviruses, K1 and STP exhibit no similarity in amino acid sequence or in structural organization Since STP is required for the oncogenic potential of HVS, we investigated the functional consequence of K1 expression Expression of the K1 gene in rodent fibroblasts produced morphologic changes and focus formation indicative of transformation A recombinant herpesvirus in which the STP oncogene of HVS was replaced with K1, immortalized primary T lymphocytes to IL-2 independent growth and induced lymphoma in common marmosets These results demonstrate the transforming potential of the K1 gene of KSHV PUBLICATIONS Lee, H, Veazey, R, Williams, K, Li, M, Guo, J, Neipel, F, Fleckenstein, B, Lackner, A, Desrosiers, RC, and Jung, JU Deregulation of cell growth by the K1 gene of Kaposi's sarcoma-associated herpesvirus Nature Med 1998; 4:435-440