MAGNESIUM STATUS & REGULATION IN HEALTHY & SICK NEONATES

镁状况

• 批准号: 6205884
• 负责人: RONALD JT CORBETT
• 金额: $ 0.7万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-15 至 2000-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6205884
• 关键词: biomedical resource; child (0-11); female; hematology; human subject; infant mortality; low birth weight infant human; male; minerals; mother /infant health care; nuclear magnetic resonance spectroscopy; technology /technique

In this project, atomic absorption spectroscopy, nuclear magnetic resonance spectroscopy, and ion selective electrodes are being used to characterize the relative fractions of magnesium in the ionized, protein-bound, and ligand-complexed pools of the blood for normal and sick preterm and term neonates as well as maternal blood and cord blood. The broad goal is to use this new technology to obtain a more complete characterization of the distribution of Mg in blood, which in turn will provide a better understanding of magnesium regulation in pregnant women, preterm and sick infants, and lead to useful new clinical information which may alter the present management of these patients. Our preliminary results demonstrate the feasibility of quantifying the Mg pools in plasma, and the potential for large variations in the size of these pools to occur in both mother and infant during and following MgSO4 therapy. The data demonstrate that the intravenous infusion of MgSO4 does not simply add Mg2+ ions to a static solution of plasma. Although it has been recognized that the kidney is the main regulatory organ of Mg homeostasis, the preliminary results suggest that plasma is capable of partially buffering acute increases in ionized Mg, probably due to a combination of Mg buffering from both high and low affinity Mg binding ligands present in blood. There are indications that the extent of this buffering increases in response to hypermagnesemia over a period of hours. (Collaborative 4) REPORT PERIOD: (09/01/97-08/31/98)

在该项目中，原子吸收光谱、核磁 共振光谱法和离子选择性电极被用于 表征电离中镁的相对分数， 蛋白质结合和配体复合的血液库，用于正常和 患病的早产儿和足月新生儿以及母体血液和脐带 血。 总体目标是利用这项新技术获得更多 完整表征血液中镁的分布，其中 反过来将有助于更好地理解镁的调节 孕妇、早产儿和患病婴儿，并带来有用的新产品 可能改变目前这些管理的临床信息 患者。 我们的初步结果证明了可行性 量化血浆中的镁池，以及大面积镁池的潜力 这些池的大小在母亲和 MgSO4 治疗期间和之后的婴儿。 数据表明 静脉输注 MgSO4 并不是简单地将 Mg2+ 离子添加到 血浆静态溶液。 尽管人们已经认识到， 肾脏是镁稳态的主要调节器官，初步 结果表明血浆能够部分缓冲急性 离子化镁的增加，可能是由于镁缓冲的组合 来自血液中存在的高亲和力和低亲和力镁结合配体。 有迹象表明，这种缓冲的程度在 数小时内对高镁血症的反应。 （合作4） 报告期：(09/01/97-08/31/98)