MAGNESIUM STATUS & REGULATION IN HEALTHY & SICK NEONATES

镁状况

• 批准号: 6335256
• 负责人: RONALD JT CORBETT
• 金额: $ 0.7万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2001-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6335256
• 关键词: biomedical resource; child (0-11); female; hematology; human subject; infant mortality; low birth weight infant human; male; minerals; mother /infant health care; nuclear magnetic resonance spectroscopy; technology /technique

In this project, atomic absorption spectroscopy, nuclear magnetic resonance spectroscopy, and ion selective electrodes are being used to characterize the relative fractions of magnesium in the ionized, protein-bound, and ligand-complexed pools of the blood for normal and sick preterm and term neonates as well as maternal blood and cord blood. The broad goal is to use this new technology to obtain a more complete characterization of the distribution of Mg in blood, which in turn will provide a better understanding of magnesium regulation in pregnant women, preterm and sick infants, and lead to useful new clinical information which may alter the present management of these patients. Our preliminary results demonstrate the feasibility of quantifying the Mg pools in plasma, and the potential for large variations in the size of these pools to occur in both mother and infant during and following MgSO4 therapy. The data demonstrate that the intravenous infusion of MgSO4 does not simply add Mg2+ ions to a static solution of plasma. Although it has been recognized that the kidney is the main regulatory organ of Mg homeostasis, the preliminary results suggest that plasma is capable of partially buffering acute increases in ionized Mg, probably due to a combination of Mg buffering from both high and low affinity Mg binding ligands present in blood. There are indications that the extent of this buffering increases in response to hypermagnesemia over a period of hours. (Collaborative 4) REPORT PERIOD: (09/01/97-08/31/98)

在这个项目中，原子吸收光谱，核磁共振， 共振光谱，离子选择性电极被用来 表征镁在离子化的， 蛋白质结合的和配体复合的血液池， 患病早产儿和足月新生儿以及母体血液和脐带血 血 广泛的目标是利用这项新技术获得更多的 完整表征血液中镁的分布，这在 反过来将提供一个更好的了解镁的规定， 孕妇、早产儿和患病婴儿，并导致有用的新的 临床信息，这可能会改变目前的管理，这些 患者 我们的初步结果表明， 定量血浆中的Mg池，以及大的 这些池的大小变化发生在母亲和 在MgSO4治疗期间和之后的婴儿。 数据证明 MgSO4的静脉输注不是简单地将Mg2+离子添加到 等离子体静态溶液。 虽然已经认识到， 肾脏是镁稳态的主要调节器官， 结果表明，血浆能够部分缓冲急性 离子化镁的增加，可能是由于镁缓冲 来自血液中存在的高和低亲和力Mg结合配体。 有迹象表明，这种缓冲的程度增加， 在数小时内对高镁血症的反应。 （合作4） 报告期：（一九九七年九月一日至一九九八年八月三十一日）