TRAINING IN USE OF DMX ELECTRONICS & NMR IMAGING

DMX 电子设备使用培训

• 批准号: 6120943
• 负责人: SUNDARAM GUNASEKARAN
• 金额: $ 0.13万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120943
• 关键词: biomedical resource; genetics; nuclear magnetic resonance spectroscopy; nutrition; nutrition related tag; plants

Carotenoids have been shown to be important both nutritionally and medicinally. Carotenoid accumulation was compared during growth and storage of two near isolines of carrots, YYy1y1y2y2RPRP and rprp. These genotypes exhibit orange and pale-range roots respectively. The RP gene reduces total carotenoid content by 93%. It was suspected that RP gene only affects the synthesis of ?- and ?-carotenes. Total carotenoid content were quantified using HPLC and spectrophotometric analyses. HPLC analysis at lambda=296nm revealed two large unique peaks in rprp with elution times of 27.5 to 28.5 minutes that were of lesser abundance in YYy1y1y2y2yRPPR, suggesting that the rate of ?-and ?-carotene accumulation is not the only difference between the two near isolines. Compounds that elute around 27.5 to 28.5 minutes will be identified using mass spectrometry and NMR.

类胡萝卜素已被证明在营养和健康方面都很重要