Establishment of International Plant and Insect Pathogen Sequence Database (IPIPSD) Using Existing Deep Sequencing Data

这是一个国际合作项目，旨在利用现有的数据集使用新的生物信息学工具的病原体筛选和鉴定。现有的数据集是由两个地区领先的国际财团在1000植物（1KP）转录组和1000昆虫转录组进化（1KITE）项目中新生成的。正在进行的生物信息学分析由中国国家基因库（CNGB）协调。用于病原体筛查的新技术工具是在先前NERC资助的项目中建立的，该项目是在技术概念验证计划中向PI提供的。新的机会是基于使用两个大型测序数据集和新的生物信息学管道进行病原体筛选研究的共同利益。目标是建立一个国际植物和昆虫病原体数据库（IPIPSD），该数据库可以对下一代测序（NGS）技术生成的数据集中的病原体序列进行存档、分类和表示。1KP和1KITE项目旨在发现生活在各种环境/生态位中的非模式物种的基因和基因功能。鸟枪NGS测序策略无差别地产生源自植物/昆虫以及天然感染它们的病原体的序列。我们建议从1KP和1KITE文库中搜索、收获、注释和排序病原体序列。IPIPSD将主要由非模式物种组成，因此对其他现有数据库来说是独一无二的。该数据库将在同行评审的科学期刊上发表，并通过万维网向公众免费开放。为了宣传IPIPSD和推广病原体筛查技术，2014年11月第九届国际基因组学会议提议举办病原体筛查国际研讨会。一旦建立，IPIPSD将促进环境样本中的病原体筛查，为支持环境/生态研究提供急需的知识进步。此外，IPIPSD还将作为一个大型长期国际合作的平台，例如，基因组10000（基因组10K）和5000昆虫和节肢动物基因组测序计划（i5K）也在CNGB中托管。

This is an international collaboration project aiming to exploit existing datasets using new bioinformatics tools of pathogen screening and identification. The existing datasets are newly generated by two area-leading international consortia in the 1000 Plant (1KP) transcriptome and 1000 Insect Transcriptome Evolution (1KITE) projects. Ongoing bioinformatics analyses are co-ordinate by the China National Genebank (CNGB). The newly available technology tool for pathogen screening is established in a previous NERC funded project to the PI in the Technology Proof of Concept programme. The new opportunity is based on the mutual benefits for conducting a pathogen screening study using the two large sequencing datasets and the new bioinformatics pipeline. The objective is to set up an International Plant and Insect Pathogen Database (IPIPSD) that can archive, sort and represent pathogen sequences from datasets generated by the Next Generation Sequencing (NGS) technology. The 1KP and 1KITE projects were designed to make gene and gene function discoveries in non-model species living in a wide variety of environmental/ecological niches. The shoot gun NGS sequencing strategy non-discriminately produced sequences derived from plants/insects as well as from pathogens naturally infected them. We propose to search, harvest, annotate and sort pathogen sequences from the 1KP and 1KITE libraries. IPIPSD will be largely composed of non-model species thus is unique to the other existing databases. The database will be published in peer reviewed scientific journals and made freely accessible to the public via the World Wide Web. To publicise IPIPSD and to promote the pathogen screening technology, an International Workshop of Pathogen Screening is proposed in the 9th International Conference of Genomics in November 2014. Once set up, IPIPSD will facilitate pathogen screening in environmental samples, providing a much needed knowledge advance for supporting environmental/ecological studies. Furthermore, IPIPSD will serve as a platform for large long term international collaborations, e.g., the Genome 10000 (Genome 10K) and 5000 Insect and Arthropod Genome Sequencing Initiative (i5K) which are also hosted in CNGB.