BONE CELL DIFFERENTIATION VIA CELL ADHESION
BONE CELL DIFFERENTIATION VIA CELL ADHESION
批准号:
6235700
负责人:
LOUIS V AVIOLI
金额:
$22.75万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-04-01 至 1998-08-09
关键词:
biomarker cell adhesion cell adhesion molecules cell cell interaction cell differentiation cell growth regulation extracellular matrix gap junctions gene expression human tissue immunofluorescence technique immunoprecipitation in situ hybridization integrins laboratory rat northern blottings nucleic acid probes osteoblasts osteogenesis polymerase chain reaction protein biosynthesis tissue /cell culture western blottings
中文摘要
骨形成是一个由成骨细胞和a细胞介导的多步骤过程
英文摘要
Bone formation is a multistep process mediated by the osteoblast and a
variety of circulating endocrine factors in addition to local non-
hormonal substances which function to control osteoblast growth and
differentiation via paracrine and autocrine modes. It has been well-
established that hormonal and non-hormonal effects on osteogenesis are
mediated by signals which are transmitted from the outside to the inside
of cells in the response to ligand binding, which stimulates second
messengers and the ultimate transcription of those genes essential to
coordinate the growth and differentiation of the osteoblast. Biological
signals can also be transmitted from the inside of cells modulating the
binding affinity of cell adhesion molecules as well as between cells via
gap junctions. In fact early observations which demonstrated that bone
cells can adhere to a variety of extracellular matrix substrates (ECM)
have now been extended to reveal that a variety of integrins are
expressed by osteoblasts, and that the integrin-ECM interaction is a
required, immediate event in the transduction of biological signals which
condition the osteoblast response to trophic factors. Moreover, cell
adhesions molecules (CAMs) have also been identified in osteoblasts
during embryogenesis. Consequently, we plan to pursue the hypothesis
that cell-cell and cell-matrix contact are essential for the transduction
of those biological events which are essential to initiate and modulate
the orderly sequential expression of osteoblastic traits. To achieve
this goal we will analyze the expression and synthesis of cell matrix
(integrins) and CAMs in human and rodent osteoblastic cell lines as well
as in normal primary cultures. Our experiments are all designed to
analyze the mechanism by which cell adhesion molecules regulate
osteoblast differentiation utilizing osteoprogenitor marrow stromal cell
populations and specific biological markers of the phenotypic expression
of osteoblast differentiation. The effects of calcitropic hormones and
other osteotropic factors on the expression and function of the CAMs will
be evaluated, as well as the correlation between the regulation of
osteoblast differentiation by osteotrophic factors and their effect on
cell-cell contact. Results of these studies should provide important
insight into those regulatory processes which are essential for
osteoblast differentiation into bone forming (mineralizing) cells, and
thus facilitate potential exploitation of these properties for the
stimulation of osteoblastic development and function in vitro or
osteogenesis in vivo.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
BONE CELL DIFFERENTIATION VIA CELL ADHESION
-
批准号:6338642
-
项目类别:
-
资助金额:$24.58万
-
财政年份:2000
-
负责人:LOUIS V AVIOLI
-
依托单位:
BONE CELL DIFFERENTIATION VIA CELL ADHESION
-
批准号:6100404
-
项目类别:
-
资助金额:$24.58万
-
财政年份:1999
-
负责人:LOUIS V AVIOLI
-
依托单位:
STUDIES OF METABOLIC BONE DISEASE
-
批准号:6112925
-
项目类别:
-
资助金额:$2.44万
-
财政年份:1998
-
负责人:LOUIS V AVIOLI
-
依托单位:
BIOLOGY OF CELL/CELL COMMUNICATION IN BONE
-
批准号:2675862
-
项目类别:
-
资助金额:$126.39万
-
财政年份:1998
-
负责人:LOUIS V AVIOLI
-
依托单位:
BONE CELL DIFFERENTIATION VIA CELL ADHESION
-
批准号:6268336
-
项目类别:
-
资助金额:$25.28万
-
财政年份:1998
-
负责人:LOUIS V AVIOLI
-
依托单位:
STUDIES OF METABOLIC BONE DISEASE
-
批准号:6244115
-
项目类别:
-
资助金额:$2.25万
-
财政年份:1997
-
负责人:LOUIS V AVIOLI
-
依托单位:
STUDIES OF METABOLIC BONE DISEASE
-
批准号:6274159
-
项目类别:
-
资助金额:$2.24万
-
财政年份:1997
-
负责人:LOUIS V AVIOLI
-
依托单位:
CALCIUM TRANSPORT, BONE CELL FUNCTION, AND AGING
-
批准号:3092361
-
项目类别:
-
资助金额:$97.87万
-
财政年份:1983
-
负责人:LOUIS V AVIOLI
-
依托单位:
DIABETES, KIDNEY, CALCIUM TRANSPORT, AND AGING
-
批准号:3092360
-
项目类别:
-
资助金额:$37.54万
-
财政年份:1983
-
负责人:LOUIS V AVIOLI
-
依托单位:
CALCIUM TRANSPORT, BONE CELL FUNCTION, AND AGING
-
批准号:3092367
-
项目类别:
-
资助金额:$93.67万
-
财政年份:1983
-
负责人:LOUIS V AVIOLI
-
依托单位:
DIABETES, KIDNEY, CALCIUM TRANSPORT, AND AGING
-
批准号:3092364
-
项目类别:
-
资助金额:$44.98万
-
财政年份:1983
-
负责人:LOUIS V AVIOLI
-
依托单位:
CALCIUM TRANSPORT, BONE CELL FUNCTION, AND AGING
-
批准号:3092366
-
项目类别:
-
资助金额:$104.84万
-
财政年份:1983
-
负责人:LOUIS V AVIOLI
-
依托单位:
BIOLOGY OF CELL-CELL COMMUNICATION IN BONE
-
批准号:2078811
-
项目类别:
-
资助金额:$109.29万
-
财政年份:1983
-
负责人:LOUIS V AVIOLI
-
依托单位:
BIOLOGY OF CELL-CELL COMMUNICATION IN BONE
-
批准号:2390489
-
项目类别:
-
资助金额:$113.75万
-
财政年份:1983
-
负责人:LOUIS V AVIOLI
-
依托单位:
CALCIUM TRANSPORT, BONE CELL FUNCTION, AND AGING
-
批准号:3092368
-
项目类别:
-
资助金额:$99.72万
-
财政年份:1983
-
负责人:LOUIS V AVIOLI
-
依托单位:
BIOLOGY OF CELL-CELL COMMUNICATION IN BONE
-
批准号:2078810
-
项目类别:
-
资助金额:$107.0万
-
财政年份:1983
-
负责人:LOUIS V AVIOLI
-
依托单位:
CALCIUM TRANSPORT, BONE CELL FUNCTION, AND AGING
-
批准号:3092365
-
项目类别:
-
资助金额:$98.11万
-
财政年份:1983
-
负责人:LOUIS V AVIOLI
-
依托单位:
CALCIUM TRANSPORT, BONE CELL FUNCTION, AND AGING
-
批准号:3092362
-
项目类别:
-
资助金额:$3.45万
-
财政年份:1983
-
负责人:LOUIS V AVIOLI
-
依托单位:
DIABETES, KIDNEY, CALCIUM TRANSPORT, AND AGING
-
批准号:3092363
-
项目类别:
-
资助金额:$43.56万
-
财政年份:1983
-
负责人:LOUIS V AVIOLI
-
依托单位:
BIOLOGY OF CELL-CELL COMMUNICATION IN BONE
-
批准号:2078809
-
项目类别:
-
资助金额:$104.33万
-
财政年份:1983
-
负责人:LOUIS V AVIOLI
-
依托单位:
国内基金
海外基金
GMFG/F-actin/cell adhesion 轴驱动 EHT 在造
血干细胞生成中的作用及机制研究
-
批准号:TGY24H080011
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2024
-
负责人:李鸿鹄
-
依托单位: