CORE--CYTOKINES

细胞因子和细胞表型核心将提供特殊的 在检测和测量细胞因子和 细胞群体的分析和纯化。这一核心将 结合南希·斯特里特博士和路易斯·皮克博士的专业知识 提供最先进的技术来分析老鼠和 人类细胞因子和细胞群。细胞因子分析，两者都是 在蛋白质和信使核糖核酸的水平上，将继续是一个主要焦点。它 将提供：1)分析人类和老鼠的专业知识 细胞因子，2)通过酶联免疫吸附试验和/或生物测定对细胞因子进行定量， 3)采购和制备单抗所需的试剂- 特定的细胞因子分析，4)质量控制，5)指南 和培训国家发改委调查人员建立人权 细胞因子分析。我们将特别强调 人类细胞因子分析的进展。为此，ELISA 针对人类细胞因子的生物检测技术正在研发中。 此外，为了满足眼前的需求，该核心已扩展到 包括细胞表型和流式细胞术，这将是 由皮克博士执导。他的实验室将提供专业知识和 细胞群体分析和纯化的专用试剂 通过提供：1)高分辨率多参数流动实现悬浮 细胞表面和细胞内抗原的细胞学分析， 特别强调分泌抑制细胞中的细胞因子， 和细胞周期(DNA)和细胞凋亡分析，2)多参数 细胞分选，3)这些技术的培训，4)咨询 实验集-u和数据分析，以及5)协助 收购和/或生产特殊相关试剂。 人和小鼠的定量聚合酶链式反应分析 细胞因子可以通过使用内部标准DNA来实现 在皮克博士和斯特里特博士的实验室中产生的结构， 分别进行了分析。总而言之，扩大这一核心实验室将 为人类细胞因子分析和细胞提供即时支持 同时保持所有现有的小鼠的表型 细胞因子支持。

The Cytokine and Cell Phenotype Core will provide special expertise in the detection and measurement of cytokins and the analysis and purification of cell populations. This Core will combine the expertise of Drs. Nancy Street and Louis Picker to provide state-of-the-art technology in the analysis of murine and human cytokines and cell populations. Analysis of cytokines, both at protein and mRNA levels, will continue to be a major focus. It will provide: 1) expertise in analyzing human and mouse cytokines, 2) quantitation of cytokines via ELISA and/or bioassay, 3) procurement and preparation of reagents required for mono- specific cytokine assays, 4) quality of control, and 5) guidelines and training of SDRC investigators in the establishment of human cytokine assays. Particular emphasis will be given to the development of human cytokine analysis. To this end, ELISAs and bioassays specific for human cytokines are being developed. Additionally, to meet immediate needs, this core has expanded to include Cell Phenotyping and Flow Cytometry which will be directed by Dr. Picker. His laboratory will provide expertise and special reagents for analysis and purification of cell populations in suspension by providing: 1) high resolution multiparameter flow cytometric analysis of both cell surface and intracellular antigens, with particular emphasis on cytokines in secretion inhibited cells, and cell cycle (DNA) and apoptosis analysis, 2) multiparameter cell sorting, 3) training in these techniques, 4) consultation for experimental set-u and data analysis, and 5) assistance in the acquisition and/or production of special relevant reagents. Additionally, quantitative PCR analysis of human and murine cytokines is possible through use of internal standard DNA constructs generated within the labs of Drs. Picker and Street, respectively. In summary, expansion of this Core Laboratory will provide immediate support for human cytokine analysis and cell phenotyping while maintaining all currently available murine cytokine support.