CORE--FLOW CYTOMETRY

核心——流式细胞术

• 批准号: 6234926
• 负责人: SUSAN JACKSON
• 金额: $ 13.4万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-01 至 1998-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6234926
• 关键词: AIDS; biomedical facility; flow cytometry

This proposal is a request for continued partial funding of the Flow Cytometry Core Facility (FACS Core) within the UAB CFAR. The Primary purpose of the FACS Core are to make available to CFAR investigators access to the equipment, trained personnel, and expertise to perform cell analysis and purification with state-of-the-art equipment and techniques on a reasonable cost for services basis and to offer these services in a facility capable of handling potentially infectious material. The FACS Core employs a dedicated full-time experienced cytometer operator who has served about 60 users in 29 laboratories over the past year. The facility is equipped with a FACStar four parameter single laser cytometer/sorter; data handling is performed by a CONSORT 30 extended analysis computer system which incorporates two integrated Hewlett Packard HP 300 microcomputers. At present, the appropriate software to perform certain CONSORT 30-capable functions (univariate through tetravariate analysis via single or multiple histograms, contour graphs with appropriate statistics, DNA curve fitting, immune monitoring, reticulocyte/platelet analysis, and histogram overlapping with statistics software) is not available to the FACS Core. Accordingly, funding to purchase these applications is requested in this proposal. Sufficient funds are requested to insure stable salary support and continuing education for the operator and to cover the field service contract for the cytometer. The requested level of support will be sufficient to cover a significant proportion of the actual cost of operating the core and it is estimated that this will allow the present rate of $50/hour charged to Center investigators for use of the FACS Core to be sustained into the foreseeable future.

这项提议是对继续为流动提供部分资金的请求 UAB CFAR内的细胞学核心设施(FACS核心)。初选 FACS核心的目的是为CFAR调查人员提供 获得执行CELL所需的设备、经过培训的人员和专业知识 用最先进的设备和技术进行分析和纯化 以合理的服务成本为基础，并在 能够处理潜在传染性物质的设施。《FACS》 CORE雇佣了一名专职的全职经验丰富的细胞仪操作员 在过去的一年里，它为29个实验室的大约60名用户提供了服务。这个 设备配备了FACStar四参数单激光器 细胞仪/分选器；数据处理由扩展的Conort 30执行 集成了两个集成休利特的分析计算机系统 帕卡德·惠普300微型计算机。目前，相应的软件可用于 执行某些conort 30功能函数(单变量到 通过单个或多个直方图、等高线图进行四变量分析 有了适当的统计，DNA曲线拟合，免疫监测， 网织红细胞/血小板分析，直方图与统计重叠 软件)不适用于FACS核心。因此，资金将用于 本建议书中要求购买这些应用程序。足够 要求提供资金以确保稳定的工资支持和持续 对操作员进行培训，并涵盖以下方面的现场服务合同 细胞仪。所请求的支持水平将足以 支付堆芯实际运营成本的很大一部分 据估计，这将使目前的费率为每小时50美元 向中心调查人员收取费用，以维持FACS核心的使用 进入可预见的未来。