Biogeochemical cycling of N-osmolytes in the surface ocean

表层海洋中氮渗透剂的生物地球化学循环

• 批准号: NE/M002233/1
• 负责人: Yin Chen
• 金额: $ 45.31万
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FM002233%2F1
• 关键词: Biogeochemical; cycling; osmolytes; surface; ocean

Nitrogen-containing compounds, including glycine betaine (GBT), choline and trimethylamine N-oxide (TMAO) are ubiquitous in marine organisms. They are used by marine organisms as compatible solutes in response to changes in environmental conditions, such as increasing salinity, because they do not interfere with cell metabolism. They also have beneficial effects in protecting proteins against denaturation due to chemical or physical damage.In the marine environment, these compounds are frequently released from these organisms directly into seawater due to changing environmental conditions, such as by viral lysis or grazing. The released nitrogenous osmolytes serve as important nutrients for marine microorganisms, which can use them as carbon, nitrogen and energy sources. It is well known that the degradation of these nitrogenous osmolytes contribute to the release of climate-active gases, including volatile methylated amines. Methylated amines are important sources of aerosols in the marine atmosphere, which help to reflect sunlight and cause a cooling effect on the climate. There is an urgent need to understand the microbial metabolism of these compounds and their seasonal cycles in the marine water column, in order to better understand their role in marine biogeochemical cycles and their role in future climate change.Built on the recent progress of the discovery of a new pathway of TMAO degradation in marine organisms and the development of a powerful liquid chromatography with mass spectrometry (LC-ESI-MS) method for simultaneous quantification of these nitrogenous osmolytes from the applicants' laboratories, this timely proposal aims to determine the seasonal cycle of nitrogenous osmolytes in surface seawater and to address how these environmentally-relevant compounds are degraded and what are the major microorganisms that are involved in the process. The data generated will fill in a major gap in our knowledge of the marine carbon and nitrogen cycles and the contribution of these compounds in future climate change through the release of climate-active molecules. Using the newly developed analytic techniques, we aim to determine the seasonal cycle of standing concentrations of nitrogenous osmolytes in the surface seawater and microbial oxidation activities. These data will be incorporated into a biogeochemical model for future prediction of biogeochemical cycles of N-osmolytes under climate change. Using cultivated model organisms, we aim to define the key genes, enzymes and the metabolic pathways in GBT and TMAO degradation by marine planktonic microbes.Using molecular and single cell manipulation techniques, we aim to further determine the key microbial players involved in the metabolism of nitrogenous osmolytes in surface seawater from the English Channel.This work will generate novel knowledge about our understanding of the microbial transformation of these nitrogen containing compounds, and will fill a serious gap in knowledge of marine carbon and nitrogen cycles. The project is expected to further strengthen the UK as a leading country not only in research of marine biogeochemical cycles and marine microbiology, but also in the development of cutting edge technology in environmental science.

含氮化合物，包括甘氨酸甜菜碱(GBT)、胆碱和三甲胺N-氧化物(TMAO)广泛存在于海洋生物中。它们被海洋生物用作相容的溶质，以应对环境条件的变化，如盐度的增加，因为它们不会干扰细胞新陈代谢。在海洋环境中，由于环境条件的变化，如病毒溶解或放牧，这些化合物经常从这些生物直接释放到海水中。释放的含氮渗透分子是海洋微生物的重要营养物质，可作为碳源、氮源和能源利用。众所周知，这些含氮渗透分子的降解有助于释放气候活性气体，包括挥发性甲基化胺。甲基胺是海洋大气中气溶胶的重要来源，有助于反射阳光并对气候造成降温效应。迫切需要了解这些化合物在海洋水柱中的微生物代谢及其季节循环，以便更好地了解它们在海洋生物地球化学循环中的作用以及它们在未来气候变化中的作用。基于最近发现海洋生物中TMAO降解的新途径的进展，以及申请者实验室开发出一种同时定量这些含氮渗透分子的高效液相色谱-质谱仪(LC-ESI-MS)方法，这项适时的提案旨在确定表层海水中含氮渗透分子的季节循环，并解决这些与环境相关的化合物是如何降解的，以及参与这一过程的主要微生物有哪些。产生的数据将填补我们关于海洋碳和氮循环以及这些化合物通过释放气候活性分子在未来气候变化中的贡献的知识的一个主要空白。利用新开发的分析技术，我们的目标是确定表层海水中含氮渗透分子的驻留浓度和微生物氧化活动的季节周期。这些数据将被纳入生物地球化学模型，用于未来预测气候变化下N-渗透分子的生物地球化学循环。利用培养的模式生物，我们的目标是确定海洋浮游微生物降解GBT和TMAO的关键基因、酶和代谢途径；我们的目标是利用分子和单细胞操作技术，进一步确定参与英国频道表层海水中含氮渗透分子代谢的关键微生物角色。这项工作将为我们理解这些含氮化合物的微生物转化提供新的知识，并将填补海洋碳氮循环知识的严重空白。该项目预计将进一步加强英国作为领先国家的地位，不仅在海洋生物地球化学循环和海洋微生物学研究方面，而且在环境科学尖端技术的发展方面也是如此。

项目成果

Aminolipids elicit functional trade-offs between competitiveness and bacteriophage attachment in Ruegeria pomeroyi.

• DOI: 10.1038/s41396-022-01346-0
• 发表时间: 2023-03
• 期刊: ISME JOURNAL
• 影响因子: 11
• 作者: Stirrup, Rachel;Mausz, Michaela A.;Silvano, Eleonora;Murphy, Andrew;Guillonneau, Richard;Quareshy, Mussa;Rihtman, Branko;Ferretjans, Maria Aguilo;He, Ruo;Todd, Jonathan D.;Chen, Feng;Scanlan, David J.;Chen, Yin
• 通讯作者: Chen, Yin

Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation.

• DOI: 10.3389/fmicb.2020.552135
• 发表时间: 2020
• 期刊: Frontiers in microbiology
• 影响因子: 5.2
• 作者: Silvano E;Yang M;Wolterink M;Giebel HA;Simon M;Scanlan DJ;Zhao Y;Chen Y
• 通讯作者: Chen Y

Lipid remodelling is a widespread strategy in marine heterotrophic bacteria upon phosphorus deficiency.

• DOI: 10.1038/ismej.2015.172
• 发表时间: 2016-04
• 期刊: The ISME journal
• 作者: Sebastián M;Smith AF;González JM;Fredricks HF;Van Mooy B;Koblížek M;Brandsma J;Koster G;Mestre M;Mostajir B;Pitta P;Postle AD;Sánchez P;Gasol JM;Scanlan DJ;Chen Y
• 通讯作者: Chen Y

Comparative genomics and mutagenesis analyses of choline metabolism in the marine Roseobacter clade.

• DOI: 10.1111/1462-2920.12943
• 发表时间: 2015-12
• 期刊: Environmental microbiology
• 影响因子: 5.1
• 作者: Lidbury I;Kimberley G;Scanlan DJ;Murrell JC;Chen Y
• 通讯作者: Chen Y

Identification of dimethylamine monooxygenase in marine bacteria reveals a metabolic bottleneck in the methylated amine degradation pathway.

• DOI: 10.1038/ismej.2017.31
• 发表时间: 2017-07
• 期刊: The ISME journal
• 作者: Lidbury I;Mausz MA;Scanlan DJ;Chen Y
• 通讯作者: Chen Y