Towards a systematic understanding of aerobic methylated amine metabolism in the ocean

系统地了解海洋中有氧甲基化胺代谢

• 批准号: NE/H016236/1
• 负责人: Yin Chen
• 金额: $ 40.09万
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2010
• 资助国家: 英国
• 起止时间: 2010 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FH016236%2F1
• 关键词: Towards; systematic; understanding; aerobic; methylated

Methylated amine compounds (MAs) are ubiquitous in the ocean and play significant roles in marine nutrient cycling and atmospheric chemistry. MAs are involved in the formation of aerosols and neutralization of atmospheric acidity resulting from the oxidation of nitrogen- and sulfur-containing compounds. MAs also contribute to the 'greenhouse effect' by the formation of nitrous oxide (N2O) and hydrogen cyanide (HCN) from their oxidation by hydroxyl radicals and ozone, and by being precursors for methane (CH4) produced by methanogens. Despite the global importance of marine MAs, modelling and quantifying their environmental impacts has been hindered by a paucity of data on the origins and sinks of these compounds. Microorganisms are known to use MAs as sources of energy and nutrients therefore affecting the concentrations of MAs in the ocean and hence fluxes across the air-sea interface; however, the identities of these key players and metabolic pathways remain largely unknown. The objective of this proposed research is to characterize the diversity and abundance of marine MA utilizers and to investigate the enzymology and regulation of MA-metabolizing pathways using model marine microorganisms. This research will test the hypothesis that MAs are used as a carbon (C) source by specialized methylotrophs (bacteria that use one-carbon (C1) compounds) in the ocean, and that diverse marine microorganisms can metabolize MAs as a source of nitrogen (N).

甲基化胺化合物（MA）在海洋中普遍存在，在海洋养分循环和大气化学中发挥着重要作用。 MA 参与气溶胶的形成以及含氮和含硫化合物氧化产生的大气酸度的中和。 MA 还通过羟基自由基和臭氧的氧化形成一氧化二氮 (N2O) 和氰化氢 (HCN)，以及作为产甲烷菌产生的甲烷 (CH4) 的前体，从而导致“温室效应”。尽管海洋 MA 具有全球重要性，但由于缺乏有关这些化合物的起源和汇的数据，对其环境影响进行建模和量化受到了阻碍。众所周知，微生物利用 MA 作为能量和营养物质的来源，因此影响海洋中 MA 的浓度，从而影响气海界面的通量；然而，这些关键参与者的身份和代谢途径仍然很大程度上未知。本研究的目的是表征海洋 MA 利用者的多样性和丰富度，并利用海洋微生物模型研究 MA 代谢途径的酶学和调节。这项研究将检验以下假设：MA 被海洋中专门的甲基营养菌（使用一碳 (C1) 化合物的细菌）用作碳 (C) 源，并且多种海洋微生物可以将 MA 代谢为氮 (N) 源。

项目成果

Handbook of Molecular Microbial Ecology I - Metagenomics and Complementary Approaches

分子微生物生态学手册 I - 宏基因组学和补充方法

• DOI: 10.1002/9781118010518.ch55
• 发表时间: 2011
• 作者: Chen Y