Towards a systematic understanding of aerobic methylated amine metabolism in the ocean

系统地了解海洋中有氧甲基化胺代谢

• 批准号: NE/H016236/1
• 负责人: Yin Chen
• 金额: $ 40.09万
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2010
• 资助国家: 英国
• 起止时间: 2010 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FH016236%2F1
• 关键词: Towards; systematic; understanding; aerobic; methylated

Methylated amine compounds (MAs) are ubiquitous in the ocean and play significant roles in marine nutrient cycling and atmospheric chemistry. MAs are involved in the formation of aerosols and neutralization of atmospheric acidity resulting from the oxidation of nitrogen- and sulfur-containing compounds. MAs also contribute to the 'greenhouse effect' by the formation of nitrous oxide (N2O) and hydrogen cyanide (HCN) from their oxidation by hydroxyl radicals and ozone, and by being precursors for methane (CH4) produced by methanogens. Despite the global importance of marine MAs, modelling and quantifying their environmental impacts has been hindered by a paucity of data on the origins and sinks of these compounds. Microorganisms are known to use MAs as sources of energy and nutrients therefore affecting the concentrations of MAs in the ocean and hence fluxes across the air-sea interface; however, the identities of these key players and metabolic pathways remain largely unknown. The objective of this proposed research is to characterize the diversity and abundance of marine MA utilizers and to investigate the enzymology and regulation of MA-metabolizing pathways using model marine microorganisms. This research will test the hypothesis that MAs are used as a carbon (C) source by specialized methylotrophs (bacteria that use one-carbon (C1) compounds) in the ocean, and that diverse marine microorganisms can metabolize MAs as a source of nitrogen (N).

甲基胺类化合物广泛存在于海洋中，在海洋营养循环和大气化学中发挥着重要作用。由于含氮和含硫化合物的氧化，MA参与了气溶胶的形成和大气酸性的中和。甲基丙烯酸甲酯还通过羟基自由基和臭氧氧化生成一氧化二氮(N2O)和氰化氢(HCN)，以及作为产甲烷菌产生甲烷(CH4)的前体，从而促成“温室效应”。尽管海洋MA具有全球重要性，但由于缺乏关于这些化合物的起源和汇的数据，对其环境影响进行建模和量化一直受到阻碍。众所周知，微生物利用mAs作为能量和营养物质的来源，从而影响海洋中mAs的浓度，从而影响海气界面的通量；然而，这些关键参与者和代谢途径的身份在很大程度上仍不清楚。这项拟议研究的目的是描述海洋MA利用者的多样性和丰富性，并利用海洋微生物模型研究MA代谢途径的酶学和调控。这项研究将检验这样一种假设，即海洋中专门的甲基营养细菌(使用一碳(C1)化合物的细菌)将Mas用作碳(C)源，以及不同的海洋微生物可以将Mas代谢为氮(N)的来源。

项目成果

Handbook of Molecular Microbial Ecology I - Metagenomics and Complementary Approaches

分子微生物生态学手册 I - 宏基因组学和补充方法

• DOI: 10.1002/9781118010518.ch55
• 发表时间: 2011
• 作者: Chen Y