CORE--LABORATORY

实验室核心提供人员、专业知识和方法，以 测量和量化各种介质、蛋白质和细胞 是国际儿童权利中心的目标和项目的核心。汇聚了 将这些技术集中在一个中心设施中，可确保分析质量 控制、数据检索和数据分析将由几个 在必要的领域具有成熟专业知识的个人 儿童权利与发展中心的项目。大多数方法都在核心中到位 调查人员的实验室。具体地说，两种组胺方法是 适用于LTC4和PGD2的RIA也可用。化验、血清白蛋白、激动素和 纤维蛋白原是公认的，肺和鼻腔的方法学也是如此。 流体的检索和分析以及定量的挑战 细胞大量涌入。核心调查人员对特异性血清IgE进行了检测 和免疫球蛋白抗体，并有新开发的IgM、IgA和SIgA的ELISA。 几种前列腺素(如PGD2、9α-11βPGF2、 PGE2等)建立了气相色谱-质谱仪(GCMS)。其他GCMS IDCR中的项目所需的技术已由 核心调查人员将测量LTB4，尿甲基组胺，以及 皮质醇/皮质醇已就位。核心调查员已经开发出 “内部”检测几种细胞因子，包括白介素4、5、6、8 和干扰素-伽马。商业检测通常用于其他细胞因子。 在必要的时候。RT-PCR技术也已经到位，并将用于 细胞内流和激活的分析。

The Laboratory Core provides the personnel, expertise and methodologies to measure and quantitate the various mediators, proteins and cells which are, central to the goals and projects in the IDCRC. The aggregation of these technologies to a central facility ensures that assay quality control, data retrieval and data analysis will be done by several individuals with established expertise in the areas necessary for the IDCRC projects. Most of the methodologies are in place in the Core investigators' laboratories. Specifically, two histamine methods are available as are RIAs for LTC4 and PGD2. Assays, serum albumin, kinins and fibrinogen are well-established, as are methodologies for lung and nasal challenges with retrieval and analysis of fluids and quantitation of cellular influx. The Core investigators have assays for specific serum IgE and IgG antibodies, and have newly developed ELISAs for IgM, IgA and sIgA. Measurements for several prostanoids (e.g., PGD2, 9alpha-11beta PGF2, PGE2, etc.) by mass spectrometry (GCMS) are established. Other GCMS technologies necessary for projects in the IDCR have been developed by Core investigators to measure LTB4, urinary methylhistamine, and cortisone/cortisol are,in place. The Core investigators have developed "in-house" assays for several cytokines, including interleukins 4,5,6,8 and INF-gamma. Commercial assays are routinely used for other cytokines when necessary. RT-PCR technology is also in place and will be used in analysis of cellular influx and activation.