FUNCTION OF NEUROACTIVE SUBSTANCES IN THE NEOSTRIATUM

新纹状体中神经活性物质的功能

• 批准号: 6243640
• 负责人: Hitoshi Kita
• 金额: $ 13万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6243640
• 关键词: 6 hydroxydopamine; Parkinson's disease; central neural pathway /tract; cooperative study; corpus striatum; denervation; disease /disorder model; dopamine; dopamine agonists; electrophysiology; electrostimulus; embryo /fetus cell /tissue; experimental brain lesion; immunocytochemistry; laboratory rat; membrane potentials; microelectrodes; nervous system transplantation; neural conduction; neuropharmacology; nontherapeutic iontophoresis; substantia nigra; synapses; tissue /cell culture; voltage /patch clamp

Degenerative loss of the nigro-striatal dopamine (DA) pathway results in deficits of sensorimotor, cognitive, and memory functions. The proposed study is designed to enhance our understanding of the roles of DA in the control of activities of striatal neurons. The study will be conducted on the rat. Some rats will receive a chemical lesion of the nigro- striatal DA pathway (i.e., an animal model of Parkinson's disease) and others will receive a lesion of the DA pathway and a subsequent graft of DA neurons (movement abnormalities are restored in these animals). The overall goals of this proposal are to determine effects of DA on electrical and pharmacological properties of the striatal neurons and to evaluate electrical and pharmacological properties of the striatal neurons that were altered after lesion of the nigro-striatal DA projections. General methods that will be used in this study include current clamp and single electrode voltage clamp recording. Intracellular labeling and retrograde labeling will be performed in order to identify the types of recorded neurons. This study is essential to the understanding of the functions of DA in the striatum. Results will shed light on the etiology of the basal ganglia disorder Parkinson's disease and may also provide information necessary for the development of more effective treatments of this disorder.

黑质-纹状体多巴胺（DA）通路的退行性丢失导致 感觉运动、认知和记忆功能的缺陷。 拟议 这项研究旨在加强我们对DA在 控制纹状体神经元的活动。 本研究的开展将 在老鼠身上。 一些老鼠会接受一种化学损伤- 纹状体DA途径（即，帕金森病的动物模型）和 其他人将接受DA通路的损伤和随后的移植， DA神经元（在这些动物中恢复运动异常）。 的 本提案的总体目标是确定DA对 纹状体神经元的电学和药理学特性， 评估纹状体的电和药理学特性 黑质-纹状体DA损伤后改变的神经元 预测。 本研究将使用的一般方法包括 电流钳和单电极电压钳记录。 细胞内标记和逆行标记将依次进行 来识别被记录的神经元的类型。 这项研究对于了解DA在 纹状体 结果将阐明基底动脉瘤的病因 神经节疾病帕金森氏病，也可能提供信息 这对于开发更有效的治疗方法是必要的。 disorder.