FUNCTION OF NEUROACTIVE SUBSTANCES IN THE NEOSTRIATUM

黑质-纹状体多巴胺(DA)通路的退行性丢失导致 感觉运动、认知和记忆功能的缺陷。建议数 这项研究旨在加深我们对DA在 纹状体神经元活动的控制。将进行这项研究 在老鼠身上。一些老鼠会受到黑质的化学损伤- 纹状体DA通路(即帕金森氏病的动物模型)和 其他人将接受DA通路的损伤和随后的移植 DA神经元(这些动物的运动异常被恢复)。这个 这项提案的总体目标是确定发展议程对 纹状体神经元的电学和药理学特性 评估纹状体的电学和药理学特性 黑质纹状体DA损毁后神经元的变化 投射。本研究将使用的一般方法包括 电流钳和单电极电压钳记录。 细胞内标记和逆行标记将按顺序进行 以确定记录的神经元的类型。 这项研究对于理解DA在脑内的作用是必不可少的。 纹状体。结果将有助于阐明基底细胞瘤的病因。 神经节障碍和帕金森氏病，也可能提供信息 对于开发更有效的治疗方法是必要的 无序。

Degenerative loss of the nigro-striatal dopamine (DA) pathway results in deficits of sensorimotor, cognitive, and memory functions. The proposed study is designed to enhance our understanding of the roles of DA in the control of activities of striatal neurons. The study will be conducted on the rat. Some rats will receive a chemical lesion of the nigro- striatal DA pathway (i.e., an animal model of Parkinson's disease) and others will receive a lesion of the DA pathway and a subsequent graft of DA neurons (movement abnormalities are restored in these animals). The overall goals of this proposal are to determine effects of DA on electrical and pharmacological properties of the striatal neurons and to evaluate electrical and pharmacological properties of the striatal neurons that were altered after lesion of the nigro-striatal DA projections. General methods that will be used in this study include current clamp and single electrode voltage clamp recording. Intracellular labeling and retrograde labeling will be performed in order to identify the types of recorded neurons. This study is essential to the understanding of the functions of DA in the striatum. Results will shed light on the etiology of the basal ganglia disorder Parkinson's disease and may also provide information necessary for the development of more effective treatments of this disorder.