EFFECT OF AGE AND AGE RELATED DISEASE ON HUMAN SALIVA

唾液是维持口腔健康的主要因素。 该项目是第一个大型的基于流行病学的研究，以记录 唾液腺功能障碍随年龄增长的患病率（具体目标1）。在 此外，还研究了两种与年龄相关的药物对唾液腺功能的影响 疾病，即，高血压及其治疗（目标2）与非胰岛素 依赖性糖尿病（NIDDM）（目标3）将被确定。本研究将 研究唾液抗菌蛋白与口腔疾病的相关性， 念珠菌状态（目标4），并将确定腮腺液分泌 与超声测量的腺体大小相关（目标5）。的 受试者是一个基于大型前瞻性社区的一部分， 圣安东尼奥NIDDM和心血管疾病的研究（即，圣 安东尼奥心脏研究和圣安东尼奥老龄化纵向研究， 自1978年以来由NIH资助的项目）。他们的年龄范围很广 (35->75岁），两个族裔群体（墨西哥裔美国人和非西班牙裔 白色），两性和三个不同的圣安东尼奥社区（低 收入、中等收入和高收入）。他们的医疗和社会经济 背景是有据可查的。将对唾液进行以下操作， 评估腺体功能：（l）未刺激和/或刺激的流速 全唾液、腮腺和颌下/舌下唾液;（2） 电解质（Na+、K+、Ca ~（2+）、Cl ~-）和（3）腺泡细胞的蛋白质 （淀粉酶、酸性和碱性富含脯氨酸的蛋白质、statherin和粘蛋白） 和导管细胞（溶菌酶和乳铁蛋白）蛋白，以及分泌 免疫球蛋白A和抗真菌蛋白，组胺素。念珠菌状态将是 通过细胞学涂片中念珠菌假菌丝的存在来确定 口腔粘膜和整个未刺激唾液中的念珠菌计数。的 本研究中待检验的假设将提供具有临床意义的 关于年龄和年龄相关疾病对唾液分泌影响的见解 非西班牙裔白人和墨西哥裔美国人的腺体功能， 第一次，对于社会经济地位较低的个人， 口腔疾病指数很高。此外，超声测量结果 将为使用这种非侵入性、低成本、非 用于临床诊断的电离辐射技术，即，转让 最先进的口腔医学技术。从以下来源获得的信息： 这项计划将有助规划非长者的口腔健康需要， 西班牙裔白色和墨西哥裔美国老年人， 我们对衰老和唾液腺病理学的临床认识 功能

Saliva is a major factor contributing to the maintenance of oral health. This project is the first large epidemiologic-based study to document the prevalence of salivary gland dysfunction with aging (SpecifiC Aim 1). In addition, the effects on salivary gland function of two age-related diseases, i.e., hypertension and its treatment (Aim 2) and non-insulin dependent diabetes (NIDDM) (Aim 3) will be determined. This study will examine the correlation of salivary antimicrobial proteins with oral candida status (Aim 4) and will determine if parotid gland fluid secretion is related to gland size as measured by ultrasonography (Aim 5). The subjects are part of a large prospective community based, cross cultural study of NIDDM and cardiovascular disease in San Antonio (i.e., The San Antonio Heart Study and The San Antonio Longitudinal Study on Aging, projects funded by NIH since 1978). They represent a broad age spectrum (35 ->75 years), two ethnic groups (Mexican American and non-Hispanic white), both genders and three distinct San Antonio neighborhoods (low income, middle-income and upper-income). Their medical and socio-economic background are well documented. The following will be done on saliva to assess gland function: (l) flow rates of unstimulated and/or stimulated whole saliva, parotid and submandibular/sublingual saliva; (2) electrolytes (Na+, K+, Ca2+, Cl-) and (3) proteins of the acinar cells (amylase, acidic and basic proline-rich proteins, statherin and mucins) and ductal cells (lysozyme and lactoferrin) proteins, as well as secretory lgA and the antifungal proteins, histatins. Candida status will be determined by the presence of candidal pseudohyphae in cytologic smears of oral mucosa and by candida counts in whole unstimulated saliva. The hypotheses to be tested in this study will provide clinically significant insights regarding the effects of age and age-related diseases on salivary gland function for both non-Hispanic whites and Mexican Americans and, for the first time, for individuals of lower socio economic status, known to have a high index of oral disease. Further, the ultrasound measurements will provide baseline data for use of this non-invasive, low-cost, non- ionizing radiation technique for clinical diagnosis, i.e., transfer of state-of-the-art technology into Oral Medicine. Information gained from this project will be useful for planning the oral health needs of non- Hispanic white and Mexican American elderly while concurrently advancing our clinical understanding of Aging and the Pathobiology of salivary gland function.