GROWTH CONE ACTIVITY AND NEURONAL PLASTICITY

该建议旨在描述细胞机制的特征， 果蝇神经元发育和可塑性的基础。 它 研究第二信使系统、膜 兴奋性、膜循环和细胞粘附，使用 遗传学生理学和细胞生物学 该提案涉及广泛的 与神经元发育和可塑性有关的各种问题。 它 包含9个具体目标，涉及的主要问题是：1） 第二信使系统在组织神经元 细胞骨架和神经肌肉接头的生理学; 2） 神经兴奋性和活动对细胞骨架的影响 组织在培养的神经元和飞行肌肉; 3）的作用， 膜囊泡蛋白在调节新的 神经元发育中的膜（例如，如生长锥中所反映的 神经突起的形成和生长速率）; 4）细胞质的作用， 酪氨酸激酶abl及其相关蛋白在细胞骨架结构中的作用 和神经元粘附。

This proposal seeks to characterize the cellular mechanisms underlying neuronal development and plasticity in Drosophila. It investigates the roles of second messenger systems, membrane excitability, membrane cycling, and cell adhesion, using methods of genetics, physiology, and cell biology. The proposal addresses a wide variety of issues relating to neuronal development and plasticity. It contains 9 specific aims, and the principal issues addressed are: 1) the role of second messenger systems in organizing the neuronal cytoskeleton and in the physiology of the neuromuscular junction; 2) the influence of nerve excitability and activity on cytoskeletal organization in cultured neurons and flight muscles; 3) the role of membrane vesicle proteins in regulating the addition of new membranes in neuronal development (e.g. as reflected in growth cone formation and neurite growth rates); 4) the role of the cytoplasmic tyrosine kinase abl and related proteins in cytoskeletal organization and neuronal adhesion.