PLASTICITY OF NEURONAL FUNCTION AND FORM

神经元功能和形式的可塑性

• 批准号: 6477324
• 负责人: CHUN-FANG WU
• 金额: $ 21.16万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6477324
• 关键词: Drosophilidae; animal genetic material tag; behavioral /social science research tag; calmodulin dependent protein kinase; cell differentiation; cyclic AMP; developmental genetics; developmental neurobiology; embryogenesis; enzyme activity; gene mutation; invertebrate embryology; larva; neural plasticity; neural transmission; neurogenesis; neurogenetics; potassium channel; synapses; tissue /cell culture

DESCRIPTION: Neurogenetic studies in Drosophila have contributed significantly to our understanding of behavioral plasticity and the underlying molecular mechanisms. This project is a continuation of our long-term efforts to bridge the gap between these two levels of approach by elucidating the associated modifications in neuronal function and structure and in neural circuit performance. A combination of genetic, molecular, morphological and physiological techniques will be employed to analyze a collection of mutants selected for distinct behavioral alterations with identified molecular defects. Previous studies from this lab have demonstrated altered neuronal firing patterns and synaptic plasticity, as well as the abnormal habituation process of an escape circuit, in the memory mutants dnc and rut with defective cAMP metabolism and in the K+channel mutants Hk and eag. This grant proposes to continue to investigate the roles of second messenger cascades at the neuronal and circuit levels, extending from the cAMP cascade (dnc and rut) to mutations that affect CaM kinase (ala), and cGMP-dependent kinase (for). Like dnc and rut, ala mutants show learning disabilities, while for exhibits altered foraging behavior. Double mutants have been constructed for analyzing effects derived from convergence and cross talk between different signal transduction pathways. In addition, K+ channels control neuronal firing properties and are known as the targets of modulation by second messenger cascades. Mutations of different K+channel subunits, Sh and slo, as well as Hk and eag, will be examined in parallel to determine how they affect synaptic plasticity and circuit performance. This study will include the habituation, a non-associative conditioning process, in the adult escape circuit, and the plasticity of synaptic efficacy and terminal sprouting in the larval neuromuscular junctions. Analysis of firing properties and synaptic function in central neurons will be facilitated by the giant neuron culture system.

描述：果蝇的神经遗传学研究做出了贡献