GROWTH CONE ACTIVITY AND NEURONAL PLASTICITY

这项提议试图描述细胞机制的特征 果蝇潜在的神经元发育和可塑性。它 研究第二信使系统、膜的作用 兴奋性、膜循环和细胞黏附，使用以下方法 遗传学、生理学和细胞生物学。该提案涉及的范围很广 与神经元发育和可塑性有关的各种问题。它 包含9个具体目标，涉及的主要问题为：1) 第二信使系统在组织神经元中的作用 细胞骨架和神经肌肉连接的生理学；2) 神经兴奋性和活性对细胞骨架的影响 培养的神经元和飞行肌肉中的组织；3) 膜泡蛋白在调节新蛋白添加中的作用 神经元发育中的膜(如生长锥体中所反映的 形成和轴突生长速度)；4)细胞质的作用 细胞骨架组织中的酪氨酸激酶ABL及其相关蛋白 和神经元粘连。

This proposal seeks to characterize the cellular mechanisms underlying neuronal development and plasticity in Drosophila. It investigates the roles of second messenger systems, membrane excitability, membrane cycling, and cell adhesion, using methods of genetics, physiology, and cell biology. The proposal addresses a wide variety of issues relating to neuronal development and plasticity. It contains 9 specific aims, and the principal issues addressed are: 1) the role of second messenger systems in organizing the neuronal cytoskeleton and in the physiology of the neuromuscular junction; 2) the influence of nerve excitability and activity on cytoskeletal organization in cultured neurons and flight muscles; 3) the role of membrane vesicle proteins in regulating the addition of new membranes in neuronal development (e.g. as reflected in growth cone formation and neurite growth rates); 4) the role of the cytoplasmic tyrosine kinase abl and related proteins in cytoskeletal organization and neuronal adhesion.