CORE--MHC MARKERS AND CELL CULTURE FACILITY

该核心是组织培养、蛋白纯化和MHC分型核心。 在该设施中，我们执行所有样品处理，包括 以下步骤：分离外周血淋巴细胞，冷冻保存， 冷冻细胞的解冻和洗涤以及B细胞系的制备。 的 核心还进行以下测试：HLA-A，B，C血清学分型， 所有新样本以及少数选定样本的HLA-DR、DQ血清学。 此外，该核心还使用以下方法对HLA II类等位基因进行DNA分型 序列特异性寡核苷酸探针杂交（SSOPH）。 其他 此外，还对新生儿进行补体蛋白分析等分型。 样品 核心还执行其他标记的额外测试， 以确认重组事件。 核心将负责 超抗原和一些单克隆抗体的纯化 在一些项目中使用的抗体。

This Core is a tissue culture, protein purification and MHC typing core. In this facility we perform all sample processing that includes the following: isolation of peripheral blood lymphocytes, cryopreservation, thawing and washing of frozen cells and preparation of B cell lines. The core also performs the following tests: HLA-A, B, C typing by serology on all new samples as well as HLA-DR, DQ serology on a few selected samples. In addition, the core does DNA typing for HLA Class II alleles using sequence specific oligonucleotide probe hybridization (SSOPH). Other typings such as complement protein analysis are also performed on new samples. The Core also performs additional tests for other markers when necessary to confirm recombinant events. The Core will be responsible for the purification of the superantigens and some of the monoclonal antibodies used in some of the projects.