CORE--TISSUE CULTURE AND MHC TYPING CORE

核心B是一个组织培养和MHC分型的核心。在这个设施里，我们 执行包括以下各项的所有样本处理；隔离 外周血淋巴细胞的冷冻保存、解冻和洗涤 冰冻细胞及B细胞系的制备。核心还执行 以下测试：对所有新样本进行血清学分型 对少数样本进行了人类白细胞抗原DR、DQ血清学检测。此外，核心 人类白细胞抗原II类等位基因的DNA分型是否使用序列特异性 寡核苷酸探针杂交技术与人类白细胞抗原-C基因分型 序列特异性引物(PCR-SSP)。其他类型，如补语 蛋白质分析，也在新的样品上进行。

Core B is a tissue culture and MHC typing Core. In this facility, we perform all sample processing that includes the following; isolation of peripheral blood lymphocytes, cryopreservation, thawing and washing of frozen cells and preparation of B cell lines. The Core also perform the following tests: HLA-A, B, C typing by serology on all new samples as well as HLA-DR, DQ serology on a few selected samples. In addition, the Core does DNA typing for HLA Class II alleles using sequence specific oligonucleotide probe hybridization (SSOPH) and DNA typing for HLA-C using sequence-specific primers (PCR-SSP). Other typings, such as complement protein analysis, are also performed on new samples.